Intravital Multiphoton Imaging of Immune Cells

  • Jo Keeble
  • Chi Ching Goh
  • Yilin Wang
  • Wolfgang Weninger
  • Lai Guan Ng
Conference paper
Part of the Advances in Intelligent and Soft Computing book series (AINSC, volume 120)

Abstract

Intravital multiphoton microscopy (MP-IVM) is a powerful imaging approach that allows direct visualization of cells within their native environment in real time. Multiphoton imaging of immune cells has been performed in different tissues, and these studies have revealed intriguing insights into the unique migratory and interactive behavior of immune cells in the steady-state and during disease conditions. Here we provide an overview of a MP-IVM model of the mouse ear skin, as well as the benefits, limitations and pitfalls of this approach. We also discuss the prospects of intravital imaging in the areas of image analysis, data management and standardization.

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Copyright information

© Springer Berlin Heidelberg 2012

Authors and Affiliations

  • Jo Keeble
    • 1
  • Chi Ching Goh
    • 1
  • Yilin Wang
    • 1
  • Wolfgang Weninger
    • 2
    • 3
    • 4
  • Lai Guan Ng
    • 1
  1. 1.Singapore Immunology Network (SIgN), Agency for Science, Technology, and Research (A*STAR)BiopolisSingapore
  2. 2.The Centenary InstituteNewtownAustralia
  3. 3.Discipline of DermatologyUniversity of SydneyCamperdownAustralia
  4. 4.Australia & Department of DermatologyRoyal Prince Alfred HospitalCamperdownAustralia

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