Nanomechanics of Yeast Surfaces Revealed by AFM

  • Etienne Dague
  • Audrey Beaussart
  • David Alsteens
Chapter
Part of the NanoScience and Technology book series (NANO)

Abstract

Despite the large and well-documented characterization of the microbial cell wall in terms of chemical composition, the determination of the mechanical properties of surface molecules in relation to their function remains a key challenge in cell biology.The emergence of powerful tools allowing molecular manipulations has already revolutionized our understanding of the surface properties of fungal cells. At the frontier between nanophysics and molecular biology, atomic force microscopy (AFM), and more specifically single-molecule force spectroscopy (SMFS), has strongly contributed to our current knowledge of the cell wall organization and nanomechanical properties. However, due to the complexity of the technique, measurements on live cells are still at their infancy.In this chapter, we describe the cell wall composition and recapitulate the principles of AFM as well as the main current methodologies used to perform AFM measurements on live cells, including sample immobilization and tip functionalization.The current status of the progress in probing nanomechanics of the yeast surface is illustrated through three recent breakthrough studies. Determination of the cell wall nanostructure and elasticity is presented through two examples: the mechanical response of mannoproteins from brewing yeasts and elasticity measurements on lacking polysaccharide mutant strains. Additionally, an elegant study on force-induced unfolding and clustering of adhesion proteins located at the cell surface is also presented.

Keywords

Atomic Force Microscopy Yeast Cell Wall Force Spectroscopy Brewing Yeast Nanomechanical Property 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by the National Foundation for Scientific Research (FNRS) and the Université Catholique de Louvain. D.A. is a postdoctoral researcher of the FNRS. E.D. is a researcher of Centre National de la Recherche Scientifique (CNRS). The authors thank Childérick Severac for careful and critical reading of the chapter.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Etienne Dague
    • 1
  • Audrey Beaussart
    • 2
  • David Alsteens
    • 2
  1. 1.CNRS UPR8001, LAASToulouseFrance
  2. 2.Institute of Condensed Matter and NanoscienceUniversité Catholique de LouvainLouvain-la-NeuveBelgium

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