Electrosynthesis and Characterisation of Antimicrobial Modified Protein Nanoaggregates

Chapter

Abstract

This chapter deals with electrochemical modification of proteins in aqueous solution leading to antimicrobial halogenated–protein nano-aggregates. It is divided into four parts. After an introduction with a presentation of biocidal N-halamine organic polymers, the first part describes the methods to obtain nanoclusters resulting from polymerization of proteins (bovine serum albumin) in the presence of electrogenerated hypochlorous and/or hypobromous acid in an aqueous solution. The substrate used to oxidize chloride and/or bromide ions is a transparent conductive film of tin dioxide deposited on glass. The second part of the chapter shows the chemical characterization by various techniques (EDX, XPS and TNB reagent) of these protein nanoclusters. The presence of chloramine and/or bromamine groups (N-halamine) is demonstrated. Next, the reaction mechanism i.e. protein polymerization and chloramine and/or bromamine formation is discussed. Finally, the capability of these clusters to prevent surface colonization by bacteria is shown. The main advantages of the synthesis described in this chapter are (1) it corresponds to a green chemistry approach, no organic solvent or toxic compounds are used, and (2) it is an easy and inexpensive process, only one step is needed to obtain a coating of antimicrobial protein nano-aggregates, notably onto a conductive SnO2 substrate.

Keywords

Bovine Serum Albumin Molecule SnO2 Film Bovine Serum Albumin Adsorb SnO2 Electrode NaBr Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Laboratoire Interfaces et Systèmes Electrochimiques, UPR 15 du CNRSUniversité P. et M. CurieParisFrance

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