Geomicrobial Manganese Redox Reactions in Metal-Contaminated Soil Substrates
Geomicrobial cycles influence metal mobilities in soil. The formation of Fe and Mn(hydr)oxides in biogeochemical horizons and the subsequent mobilization of Mn from the substrate can lead to high mobility of Mn. This process of Mn mobilization was studied in substrates derived from a former uranium mining area in column experiments. Microbially influenced manganese release was investigated in columns with an autochthonous microbial community and columns additionally inoculated with Streptomyces. Additionally, azide-poisoned columns were analyzed. Levels of 1,060 μg l−1 Mn(II) were released from inoculated columns while batch experiments led to elution of up to 28 μg l−1 Mn(II). The microbial influence on element cycling correlated with decreasing redox potentials. To study the potential of microbial reduction processes, strains isolated from these columns were investigated. One prominent bacterium, identified as Cupriavidus metallidurans, tolerated up to 30 mM Mn(II). However, no aerobic microbial Mn reduction was found indicating that Mn release was either dependent on anaerobic conditions, or microbial respiration initiated abiotic Mn reduction by decreasing the redox potential necessary for these processes.
KeywordsTotal Organic Carbon Total Organic Carbon Concentration Waste Rock Pilis Initial Total Organic Carbon Decrease Redox Potential
The authors thank Ulrike Buhler, Ines Kamp, Gundula Rudolph and Gerit Weinzierl for technical assistance. This work was supported by the German Federal Ministry of Education & Research grant no. 02S8294 KOBIOGEO and JSMC through the DFG Research Training Group 1257.
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