Dynamics of α-Helix and β-Sheet Formation Studied by Laser-Induced Temperature-Jump IR Spectroscopy

Chapter
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Part of the Biological and Medical Physics, Biomedical Engineering book series (BIOMEDICAL)

Abstract

Analysis of folding mechanisms requires a fast initiation method besides site-specific structural probes. Laser-induced temperature-jump IR spectroscopy is an ideally suited method to study α-helix and β-sheet formation in the nanosecond-to-microsecond time domain. The section gives an overview about the T-jump technique and its power to obtain site-specific dynamics by a combination with isotopic editing.

Keywords

Probe Beam Temperature Jump Stokes Line Folding Mechanism Heated Volume 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgements

The author thanks Dr. Carsten Krejtschi, former PhD student for the initial work on the spectrometer setup and various T-jump studies and Timothy A. Keiderling, University of Illinois at Chicago (USA), for inspiring collaboration and for generously providing us with isotopically labeled peptide variants.

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© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Biophysical Chemistry, Department of ChemistryUniversity of KonstanzKonstanzGermany

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