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Development of an Immunochromatographic Strip for Rapid, Instrument-Free, On-Site Detection of Uranium

  • Xiaoxia Zhu
  • Bhupal Ban
  • Xiaoxi Yang
  • Sergey S. Shevkoplas
  • Diane A. Blake
Part of the Springer Geology book series (SPRINGERGEOL)

Abstract

A one-step assay has been developed for on-site detection of environmental uranium. A monoclonal antibody with specificity for U(VI) complexed to 2,9-dicarboxyl-1,10-phenanthroline (DCP) was conjugated to colloidal gold and subsequently mixed with a sample containing U(VI) and DCP. The mixture was allowed to migrate up a nitrocellulose strip and intensity of the antibody captured at a band of immobilized U(IV)-DCP was inversely proportional to the amount of U(VI) (50–200 nM) in the sample. The strips could be read visually or the images could be captured via a cell phone camera for more quantitative analysis.

Keywords

Colloidal Gold Salmon Pink Trisodium Citrate Phone Camera Gold Chloride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Xiaoxia Zhu
    • 1
    • 2
  • Bhupal Ban
    • 1
  • Xiaoxi Yang
    • 3
  • Sergey S. Shevkoplas
    • 3
  • Diane A. Blake
    • 1
  1. 1.Department BiochemistryTulane University School of MedicineNew OrleansUSA
  2. 2.School of Public HealthNantong UniversityNantongChina
  3. 3.Department of Biomedical EngineeringTulane UniversityNew OrleansUSA

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