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Environmental Analysis of Uranium with Recombinant Antibodies

  • Diane A. Blake
  • Xiaoxia Zhu
  • Bhupal Ban
Chapter
  • 1.5k Downloads
Part of the Springer Geology book series (SPRINGERGEOL)

Abstract

The techniques of antibody phage display were used to generate antibody fragments that specifically recognized U(VI) in complex with the chelator, 2,9-dicarboxyl-1,10-phenanthroline (DCP). The single-chain fragment antibodies (scFvs) obtained by these procedures were able to bind to uranium-loaded DCP in the presence of > 1000-fold molar excess of metal-free DCP. The ability of these antibodies to tolerate high levels of chelator will enhance their ability to accurately measure uranium in groundwater that has been amended to enhance U(VI) extraction. Further engineering of these antibodies will utilize yeast display techniques.

Keywords

Competitive ELISA Recombinant Antibody scFv Antibody Phage ELISA Antibody Phage Display 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Ayriss J, Woods T, Bradbury A, Pavlik P (2007) High-throughput screening of single-chain antibodies using multiplexed flow cytometry. J Proteome Res 6: 1072–1082CrossRefGoogle Scholar
  2. Blake RC 2nd, Pavlov AR, Blake DA (1999) Automated kinetic exclusion assays to quantify protein binding interactions in homogeneous solution. Anal Biochem 272: 123–134CrossRefGoogle Scholar
  3. Blake RC 2nd, Pavlov AR, Khosraviani M, Ensley HA, Keifer GE, Yu H, Li X, Blake DA (2004) Novel monoclonal antibodies with specificity for chelated uranium(VI): Isolation and binding properties. Bioconj Chem 15: 1125–1136CrossRefGoogle Scholar
  4. Brina R, Miller AG (1993) Determination of uranium and lanthanides in real-world samples by kinetic phosphorescence analysis. Spectroscopy 8: 258–301Google Scholar
  5. Chiu YW, Chen R, Li QX, Karu AE (2000) Derivation and properties of recombinant Fab antibodies to coplanar polychlorinated biphenyls. J Agric Food Chem 48: 2614–2624CrossRefGoogle Scholar
  6. Gai SA, Wittrup KD (2007) Yeast surface display for protein engineering and characterization. Curr Opin Struct Biol 4: 467–473CrossRefGoogle Scholar
  7. Glass TR, Ohmura N, Saik H (2007) Least detectable concentration and dynamic range of three immunoassay systems using the same antibody. Anal Chem 79: 1954–1960CrossRefGoogle Scholar
  8. Hoogenboom HR (2005) Selecting and screening recombinant antibody libraries. Nature Biotechnol 23: 1105–1116CrossRefGoogle Scholar
  9. Li T, Zhang Q, Liu Y, Chen D, Hu B, Blake DA, Liu F (2006) Production of recombinant scFv antibodies against methamidophos from a phage-display library of a hyperimmunized mouse. J Agric Food Chem 54: 9085–9091CrossRefGoogle Scholar
  10. Li Y, Cockburn W, Kilpatrick J, Whitelam G (2000) High affinity scFvs from a single rabbit immunized with multiple haptens. Biochem Biophys Res Comm 268: 398–404CrossRefGoogle Scholar
  11. Melton SJ, Yu H, Williams KH, Morris SA, Long PE, Blake DA (2009) Field-based detection and monitoring of uranium in contaminated groundwater using two immunosensors. Environ Sci Technol 43: 6703–6709CrossRefGoogle Scholar
  12. Tout NL, Yau KTF, Trevors JT, Lee H, Hall JC (2001) Synthesis of ligand-specific phage-display scFv against the herbicide picloram by direct cloning from hyperimmunized mouse. J Agric Food Chem 49: 3628–3637CrossRefGoogle Scholar
  13. Yu H, Jones RM, Blake DA (2005) An immunosensor for autonomous in-line detection of heavy metals: Validation for hexavalent uranium. Int J Environ Anal Chem 85: 817–830CrossRefGoogle Scholar
  14. Zhu X, Kriegel AM, Boustany CA, Blake DA (2011) Single-chain variable fragment (scFv) antibodies optimized for environmental analysis of uranium. Anal Chem 83: 3717–3724CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Diane A. Blake
    • 1
  • Xiaoxia Zhu
    • 1
    • 2
  • Bhupal Ban
    • 1
  1. 1.Department BiochemistryTulane University School of MedicineNew OrleansUSA
  2. 2.School of Public HealthNantong UniversityNantongChina

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