Comparative Genomic Hybridisation (CGH)
Abstract
CGH is a technique used to identify unbalanced chromosomal aberrations in solid tumors. It provides a global overview of chromosomal gains and losses. Two variants are used in research and diagnostics, chromosomal CGH and array CGH. The major difference is within the detection system. In chromosomal CGH preparations of normal test chromosomes serve as the reference, on which tumor and normal DNA is competitively hybridized, whereas in array CGH either bacterial artificial clones (BAC) clones or oligonucleotides are used. Whereas in chromosomal CGH the resolution of detected aberrations is in the 10 MB range, the resolution of array CGH ranges from 1 MB to 100 bp.
Whereas in research whole genome arrays are used, for diagnostic purposes customized arrays limited to areas of interest can be designed.
Keywords
Comparative Genomic Hybridisation Array Comparative Genomic Hybridisation Metaphase Spread Comparative Genomic Hybridisation Analysis Full MediumReferences
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