A Classification Scheme for Lymphocyte Segmentation in H&E Stained Histology Images

  • Manohar Kuse
  • Tanuj Sharma
  • Sudhir Gupta
Conference paper
Part of the Lecture Notes in Computer Science book series (LNCS, volume 6388)


A technique for automating the detection of lymphocytes in histopathological images is presented. The proposed system takes Hematoxylin and Eosin (H&E) stained digital color images as input to identify lymphocytes. The process involves segmentation of cells from extracellular matrix, feature extraction, classification and overlap resolution. Extracellular matrix segmentation is a two step process carried out on the HSV-equivalent of the image, using mean shift based clustering for color approximation followed by thresholding in the HSV space. Texture features extracted from the cells are used to train a SVM classifier that is used to classify lymphocytes and non-lymphocytes. A contour based overlap resolution technique is used to resolve overlapping lymphocytes.


Lymphocytes Classification Contour Overlap Resolution 


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Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Manohar Kuse
    • 1
  • Tanuj Sharma
    • 1
  • Sudhir Gupta
    • 1
  1. 1.The LNM Institute of Information TechnologyJaipurIndia

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