Molecular Phylogenetic and Population Genetic Structuring of Macrodon sp., a Coastal and Estuarine Fish of the Western Atlantic Ocean

Chapter
Part of the Ecological Studies book series (ECOLSTUD, volume 211)

Abstract

The study on fish fauna of the Caeté estuary led to a large-scale phylogenetic analysis of one of its common fish species, Macrodon ancylodon. Phylogeographic patterns in M. ancylodon sampled from 16 locations in the subtropical and tropical western Atlantic Ocean were investigated using mitochondrial DNA 16S rRNA and cytochrome b sequences. The analysis shows two monophyletic and highly differentiated lineages of Macrodon populations in the western Atlantic Ocean, and there was no zone of overlap between the two groups, which were geographically separated in the region of South Bahia (Porto Seguro) and north Espirito Santo (Vitoria). Tropical and subtropical groups of Macrodon show nucleotide divergences for the 16S gene varying from 2.4 to 3.5%, a magnitude of genetic divergences usually observed between distinct species of Sciaenidae. The high values of divergence observed between the two groups, coupled with the clearly geographic isolation between them, strongly support our conclusions that these groups should be considered distinct species, M. ancylodon (Bloch and Schneider 1801) being the species of the tropical region (Venezuela to Bahia) and Macrodon sp. the new species of the subtropical region (Espirito Santo to Argentina). Curiously, assuming a constant rate of 1% of divergence per million years for the 16S rRNA, estimation obtained by the comparison of Panama trans-isthmian geminate species of Centropomus, we estimate that the separation between the tropical and subtropical groups of Macrodon have possibly occurred between 2.4 and 3.5 million years ago, during the late Pliocene. These genetic evidences strongly indicate a taxonomic revision of this estuarine-dependent group of fishes.

References

  1. Avise JC (1994) Molecular markers, natural history and evolution. Chapman & Hall, New YorkCrossRefGoogle Scholar
  2. Avise JC (2000) Phylogeography: the history and formation of species. Harvard University Press, Cambridge MAGoogle Scholar
  3. Beheregaray LB, Sunnucks P, Briscoe DA (2002) A rapid fish radiation associated with the last sea-level changes in southern Brazil: the silverside Odontesthes perugiae complex. Proc R Soc Lond B 269:65–73CrossRefGoogle Scholar
  4. Brunner PC, Douglas MR, Osinov A, Wilson CC, Bernatchez L (2001) Holartic phylogeography of Arctic charr (Salvelinus alpinus L.) inferred from mitochondrial DNA sequences. Evolution 55:573–586PubMedCrossRefGoogle Scholar
  5. Camargo-Zorro M (1999) Biologia e estrutura populacional das espécies da família Sciaenidae (Pisces: Perciformes), no estuário do rio Caeté município de Bragança, Pará – Brasil. MSc thesis, University of Pará, BelémGoogle Scholar
  6. Carlin JL, Robertson DR, Bowen BW (2003) Ancient divergences and recent connections in two tropical Atlantic reef fishes Epinephelus adscensionis and Rypticus saponaceous (Percoidei: Serranidae). Mar Biol 143:1057–1069CrossRefGoogle Scholar
  7. Castro BM, Miranda LBDE (1998) Physical oceanography of the western Atlantic continental shelf located between 4° N and 34° S, coastal segment (4, W). In: Robinson AR, Brink KH (eds) The sea, vol 7. Wiley, New YorkGoogle Scholar
  8. Cervigón F (1993) Los peces marinos de Venezuela, vol 2. Fundación Científica Los Roques, CaracasGoogle Scholar
  9. Chao LN (1978) A basis for classifying western Atlantic Sciaenidae (Teleostei, Perciformes). NOAA Tech Rep Circ 415:1–64Google Scholar
  10. Chenoweth SF, Hughes JM, Connolly RC (2002) Phylogeography of the pipefish, Urocampus carinirostris, suggests secondary intergradation of ancient lineages. Mar Biol 141:541–547CrossRefGoogle Scholar
  11. Colborn J, Crabtree RE, Shaklee JB, Pfeiler E, Bowen BW (2001) The evolutionary enigma of bonefishes (Albula spp.): cryptic species and ancient separations in a globally distributed shorefish. Evolution 55:807–820PubMedCrossRefGoogle Scholar
  12. Dawson MN, Staton JL, Jacobs DK (2001) Phylogeography of the tidewater goby, Eucyclogobius newberryi (Teleostei, Gobiidae), in coastal California. Evolution 55:1167–1179PubMedGoogle Scholar
  13. Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479–491PubMedGoogle Scholar
  14. Farias IP, Meyer A, Orti G (2000) Total evidence: molecules, morphology and the phylogenetics of cichlids fishes. Mol Dev Evol 288:76–92CrossRefGoogle Scholar
  15. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791CrossRefGoogle Scholar
  16. Gold JR, Richardson LR (1998) Mitochondrial DNA diversification and population structure in fishes from the Gulf of Mexico and Western Atlantic. J Hered 89:404–414CrossRefGoogle Scholar
  17. Grunwald C, Stabile J, Waldman JR, Gross R, Wirgin I (2002) Population genetics of shortnose sturgeon Acipenser brevirostrum based on mitochondrial DNA control region sequences. Mol Ecol 11:1885–1898PubMedCrossRefGoogle Scholar
  18. Gyllensten U (2006) The genetic structure of fish: differences in the intraspecific distribution of biochemical genetic variation between marine, anadromous, and freshwater species. J Fish Biol 26:691–699CrossRefGoogle Scholar
  19. Haimovici M, Martins AS, Vieira PC (1996) Distribuição e abundância de peixes teleósteos demersais sobre a plataforma continental do sul do Brasil. Rev Bras Biol 56:27–50Google Scholar
  20. Hall T (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
  21. Hickerson MJ, Ross JRP (2001) Post-glacial population history and genetic structure of the northern clingfish (Gobbiesox maeandricus), revealed from mtDNA analysis. Mar Biol 138:407–419CrossRefGoogle Scholar
  22. Isaac VJ, Braga TMP (1999) Rejeição de pescado nas pescarias da costa norte do Brasil. Arq Cienc Mar 32:39–54Google Scholar
  23. Johns WE, Lee TN, Beardsley RC, Candela J, Limeburner R, Castro B (1998) Annual cycle and variability of the North Brasil current. J Phys Oceanogr 28:103–128CrossRefGoogle Scholar
  24. Juras AA, Yamaguti N (1989) Sexual maturity, spawning and fecundity of King weakfish Macrodon ancylodon, caught off Rio Grande do Sul state (southern coast of Brazil). Bol Inst Oceanogr São Paulo 37:51–58Google Scholar
  25. Kotlík P, Bogutskaya NG, Ekmeckçi FG (2004) Circum Black sea phylogeography of Barbus freshwater fishes: divergence in the Pontic glacial refugium. Mol Ecol 13:87–95PubMedCrossRefGoogle Scholar
  26. Lowe-McConnell RH (1999) Estudos Ecológicos de Comunidades de Peixes Tropicais. Editora da Universidade de São Paulo, São PauloGoogle Scholar
  27. Mabuchi K, Nakabo T, Nishida M (2004) Molecular phylogeny of the antitropical genus Pseudolabrus (Perciformes: Labridae): evidence for a Southern Hemisphere origin. Mol Phylogenet Evol 32:375–382PubMedCrossRefGoogle Scholar
  28. Menezes NA, Figueiredo JL (1980) Manual de Peixes Marinhos do Sudeste do Brasil. IV. Teleostei (3). Museu de Zoologia da Universidade de São Paulo, São PauloGoogle Scholar
  29. Muss A, Robertson DR, Stepien CA, Wirtz P, Bowen BW (2001) Phylogeography of Ophioblennius: the role of ocean currents and geography in reef fish evolution. Evolution 55:561–572PubMedCrossRefGoogle Scholar
  30. Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New YorkGoogle Scholar
  31. Palumbi SR (1994) Genetic divergence, reproductive isolation, and marine speciation. Annu Rev Ecol Syst 25:547–572CrossRefGoogle Scholar
  32. Planes S, Doherty PJ, Bernardi G (2001) Strong genetic divergence among populations of a marine fish with limited dispersal, Acanthochromis polyacanthus, within the great barrier reef and the coral sea. Evolution 55:2263–2273PubMedGoogle Scholar
  33. Posada D, Crandall KA (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics 14:817–818PubMedCrossRefGoogle Scholar
  34. Rozas J, Sánchez-DelBarrio JC, Messeguer X, Rozas R (2003) DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics 19:2496–2497PubMedCrossRefGoogle Scholar
  35. Santos S, Schneider H, Sampaio I (2003) Genetic differentiation of Macrodon ancylodon (Sciaenidae, Perciformes) populations in Atlantic coastal waters of South America as revealed by mtDNA analysis. Genet Mol Biol 26:151–161CrossRefGoogle Scholar
  36. Santos S, Hrbek T, Farias IP, Schneider H, Sampaio I (2006) Population genetic structuring of Macrodon ancylodon (Sciaenidae) in Atlantic coastal waters of South America: deep genetic divergence without morphological change. Mol Ecol 15:4361–4373PubMedCrossRefGoogle Scholar
  37. Schneider S, Roessli D, Excoffier L (2000) Arlequin ver. 2000: a software for population genetic data analysis. Genetics and Biometry Laboratory, University of Geneva, GenevaGoogle Scholar
  38. Sinclair M (1988) Marine populations: an essay on population regulation and speciation. University of Washington Press, SeattleGoogle Scholar
  39. Stepien CA, Rosenblatt RH, Bargmeyer BA (2001) Phylogeography of the spotted sand bass, Paralabrax maculatofasciatus: divergence of Gulf of California and Pacific Coast populations. Evolution 55:1852–1862PubMedGoogle Scholar
  40. Streelman JT, Alfaro M, Westneat MW, Bellwood DR, Karl SA (2002) Evolutionary history of the parrotfishes: bio-geography, ecomorphology, and comparative diversity. Evolution 56:961–971PubMedGoogle Scholar
  41. Swofford DL (2002) PAUP* phylogenetic analysis using parsimony (* and other methods), version 4. Sinauer, Sunderland, MAGoogle Scholar
  42. Tamura F, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512–526PubMedGoogle Scholar
  43. Tang KL (2001) Phylogenetic relationships among Damselfishes (Teleostei: Pomacentridae) as determined by mitochondrial ribosomal DNA data. Copeia 3:591–601CrossRefGoogle Scholar
  44. Thompson JD, Higgins DG, Gibson TJ (1994) Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680PubMedCrossRefGoogle Scholar
  45. Thomsem H (1962) Masas de agua caracteristicas del Oceano Atlantico. Parte Sudoeste. Servicio de Hidrografía Naval, Secretaría Marina, Publ H632, Buenos AiresGoogle Scholar
  46. Tringali MD, Bert TM, Seyoum S, Bermingham E, Bartolacci D (1999) Molecular phylogenetics and ecological diversification of the transisthmian fish genus Centropomus (Perciformes: Centropomidae). Mol Phylogenet Evol 13:193–207PubMedCrossRefGoogle Scholar
  47. Vazzoler AEA de M (1963) Sobre a fecundidade e a desova da pescada-foguete. Bol Inst Oceanogr São Paulo 13:33–40CrossRefGoogle Scholar
  48. Vinson C, Gomes G, Schneider H, Sampaio I (2004) Sciaenidae fish of the Caeté River estuary, Northern Brazil: mitochondrial DNA suggests explosive radiation for the Western Atlantic assemblage. Genet Mol Biol 27:174–180CrossRefGoogle Scholar
  49. Ward RD, Woodwark M, Skibinski DOF (1994) A comparison genetic diversity levels in marine, freshwater, and anadromous fishes. J Fish Biol 44:213–232CrossRefGoogle Scholar
  50. Yamaguti N (1967) Desova da pescada-foguete, Macrodon ancylodon. Bol Inst Oceanogr São Paulo 16:101–106CrossRefGoogle Scholar
  51. Yamaguti N (1979) Diferenciação Geográfica de Macrodon ancylodon (Bloch &Schneider, 1801) na Costa Brasileira entre as Latitudes 18° 36S e 32°10S, etapa I. Bol Inst Oceanogr São Paulo 28:53–118Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Instituto de Estudos CosteirosUniversidade Federal do ParáBragançaBrazil

Personalised recommendations