In Silico AFLP: An Application to Assess What Is Needed to Resolve a Phylogeny

  • María Jesús García-Pereira
  • Armando Caballero
  • Humberto Quesada
Conference paper
Part of the Advances in Intelligent and Soft Computing book series (AINSC, volume 74)


We examined the effect of increasing the number of scored AFLP bands to reconstruct an accurate and well-supported AFLP-based phylogeny. In silico AFLP was performed using simulated DNA sequences evolving along a symmetric tree with ancient radiation. The comparison of the true tree to the estimated AFLP trees suggests that moderate numbers of AFLP bands are necessary to recover the correct topology with high bootstrap support values (i.e > 70%). However, branch length estimation is rather unreliable and does not improve substantially after a certain number of bands are sampled.


AFLP phylogeny bootstrap support simulation accuracy 


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Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • María Jesús García-Pereira
    • 1
  • Armando Caballero
    • 1
  • Humberto Quesada
    • 1
  1. 1.Departamento de Bioquímica, Genética e Inmunología, Facultad de BiologíaUniversidad de VigoVigoSpain

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