Sixty Years Research with Characean Cells: Fascinating Material for Plant Cell Biology

Chapter
Part of the Progress in Botany book series (BOTANY, volume 72)

Abstract

My nearly 60-year long and deeply rewarding research life can be divided into three periods, the first between 1952 and 1977 at Osaka University, the second between 1977 and 1990 at the University of Tokyo, and the third between 1990 and 2002 at the Fukui University of Technology. Throughout my research life, my main experimental material has been the internodal cells of the characean algae, Nitella, Chara, Nitellopsis, as well as the internodal cells of the brackish genus Lamprothamnium. The characean internodal cell is a giant cylindrical cell that is typically 50–100 mm in length and 0.5–1.0 mm in diameter (cf. my picture). The cell is characterized by a very active rotational cytoplasmic streaming with an unusually high speed amounting to 50–70 μm s−1 at 25°C as well as an exceptionally large action potential (150 mV), which can be generated by electrical, mechanical, and chemical stimuli. Due to these activities, the characean cell is often referred to the “green muscle” or the “green axon.”

Notes

Acknowledgments

I would like to express my cordial thanks to my coworkers cited above. A wide variety of productive researches mentioned above could surely not have been achieved without their cooperation.

My thanks are also due to Professor Ulrich Lüttge, the Editor of “Progress in Botany,” for his invitation to this review article, and Professor Randy Wayne, one of my former coworkers and now in the Department of Plant Physiology, Cornell University, for his kindness to read my manuscript and to give valuable suggestions in polishing the style of my English.

References

  1. Awata J, Saitoh K, Shimada K, Kashiyama T, Yamamoto K (2001) Effect of Ca2+ and calmodulin on the motile activity of characean myosin in vitro. Plant Cell Physiol 42:828–834PubMedGoogle Scholar
  2. Barry WH (1969) Coupling of excitation and cessation of cyclosis in Nitella: role of divalent cations. J Cell Physiol 72:153–160Google Scholar
  3. Benga G, Popescu O, Borza V, Pop VI, Muresan A, Mocsy I, Brain A, Wrigglesworth JM (1986) Water permeability of human erythrocytes: identification of membrane proteins involved in water transport. Eur J Cell Biol 41:252–262PubMedGoogle Scholar
  4. Bisson MA, Kirst GO (1980) Lamprothamnium, a euryhaline charophyte. II. Time course of turgor regulation. J Exp Bot 31:1237–1244Google Scholar
  5. Chen JCW, Kamiya N (1975) Localization of myosin in the internodal cells of Nitella as suggested by differential treatment with N-ethylmaleimide. Cell Struct Funct 8:109–118Google Scholar
  6. Dainty J (1963) Water relations of plant cells. Adv Bot Rev 1:279–326Google Scholar
  7. Dainty J, Ginzburg BZ (1964a) The measurement of hydraulic conductivity (osmotic water permeability to water) of internodal cells by means of transcellular osmosis. Biochim Biophys Acta 79:102–111PubMedGoogle Scholar
  8. Dainty J, Ginzburg BZ (1964b) The permeability of the cell membranes of Nitella translucens to urea and the effect of high concentrations of sucrose on this permeability. Biochim Biophys Acta 79:112–121PubMedGoogle Scholar
  9. Dainty J, Hope AB (1959) The water permeability of cells of Chara australis R. BR. Aust J Biol Sci 12:136–145Google Scholar
  10. Ding DQ, Tazawa M (1989) Influence of cytoplasmic streaming and turgor pressure gradient on the transnodal transport of rubidium and electrical conductance in Chara corallina. Plant Cell Physiol 30:739–748Google Scholar
  11. Ding DQ, Mimura T, Amino S, Tazawa M (1991a) Intercellular transport and photosynthetic differentiation in Chara corallina. J Exp Bot 42:33–38Google Scholar
  12. Ding DQ, Amino S, Mimura T, Nagata T, Tazawa M (1991b) Intercellular transport and subcellular distribution of photoassimilates in Chara corallina. J Exp Bot 42:1393–1398Google Scholar
  13. Findlay GP (1961) Voltage-clamp experiments with Nitella. Nature 191:812–814Google Scholar
  14. Findlay GP (1964) Ionic relations of cells of Chara australis. VIII. Membrane currents during a voltage clamp. Aust J Biol Sci 17:388–399Google Scholar
  15. Fujii S, Shimmen T, Tazawa M (1978) Light-induced changes in membrane potential in Spirogyra. Plant Cell Physiol 19:573–590Google Scholar
  16. Grolig F, Williamson RE, Parke J, Miller C, Anderton BH (1988) Myosin and Ca2+-sensitive streaming in the alga Chara: detection of two polypeptides reacting with a monoclonal anti-myosin and their localization in the streaming endoplasm. Eur J Cell Biol 47:22–31PubMedGoogle Scholar
  17. Hayama T, Tazawa M (1980) Ca2+ reversibly inhibits active rotation of chloroplasts in isolated cytoplasmic droplets of Chara. Protoplasma 102:1–9Google Scholar
  18. Hayama T, Shimmen T, Tazwa M (1979) Participation of Ca2+ in cessation of cytoplasmic streaming induced by membrane excitation in Characeae internodal cells. Protoplasma 99:305–321Google Scholar
  19. Higashi-Fujime S (1980) Active movement in vitro of bundles of microfilaments isolated from Nitella cells. J Cell Biol 87:569–578PubMedGoogle Scholar
  20. Higashi-Fujime S, Sumiyoshi H (2001) Molecular sliding of the fastest Chara myosin from green algae. Curr Topics Biochem Res 4:61–70Google Scholar
  21. Higashi-Fujime S, Ishikawa R, Iwasawa H, Kagami O, Kurimoto E, Kohama K, Hozumi T (1995) The fastest actin-based motor protein from the green algae, Chara, and its distinct mode of interaction with actin. FEBS Lett 375:151–154PubMedGoogle Scholar
  22. Hope AB (1961) The action potential in cells of Chara. Nature 191:811–812Google Scholar
  23. Kamitsubo E (1966) Motile protoplasmic fibrils in cells of characeae. II. Linear fibrillar structure and its bearing on protoplasmic streaming. Proc Jpn Acad 42:640–643Google Scholar
  24. Kamitsubo E (1981) Effect of supraoptimal temperature on the function of the subcortical fibrils and an endoplasmic factor in Nitella internode. Protoplasma 109:3–12Google Scholar
  25. Kamiya N (1940) The control of protoplasmic streaming. Science 92:462–463PubMedGoogle Scholar
  26. Kamiya N (1986) Cytoplasmic streaming in giant algal cells: a historical survey of experimental approaches. Bot Mag Tokyo 99:441–467Google Scholar
  27. Kamiya N (1989) My early career and the involvement of World War II. Annu Rev Plant Physiol Plant Mol Biol 40:1–18Google Scholar
  28. Kamiya N, Kuroda K (1955) Some experiments on cell amputation. In 20th Ann Meet Bot Soc Jpn, pp 46–47Google Scholar
  29. Kamiya N, Kuroda K (1956a) Artificial modification of the osmotic pressure of the plant cell. Protoplasma 46:423–436Google Scholar
  30. Kamiya N, Kuroda K (1956b) Velocity distribution of the protoplasmic streaming in Nitella cells. Bot Mag (Tokyo) 69:544–554Google Scholar
  31. Kamiya N, Kuroda K (1958) Measurement of the motive force of the protoplasmic rotation in Nitella. Protoplasma 50:144–148Google Scholar
  32. Kamiya N, Kuroda K (1973) Dynamics of cytoplasmic streaming in a plant cell. Biorheology 10:179–187PubMedGoogle Scholar
  33. Kamiya N, Tazawa M (1956) Studies on water permeability of a single cell by means of transcellular osmosis. Protoplasma 46:394–422Google Scholar
  34. Katsuhara M, Tazawa M (1986) Salt tolerance in Nitellopsis obtusa. Protoplasma 135:155–161Google Scholar
  35. Katsuhara M, Tazawa M (1987) ATP is essential for calcium-induced salt tolerance in Nitellopsis obtusa. Protoplasma 138:190–192Google Scholar
  36. Katsuhara M, Tazawa M (1988) Changes in sodium and potassium in Nitellopsis cells treated with transient salt stress. Plant Cell Environ 11:71–74Google Scholar
  37. Katsuhara M, Tazawa M (1992) Calcium-regulated channels and their bearing on physiological activities in characean cells. Phil Trans R Soc Lond B 338:19–29Google Scholar
  38. Katsuhara M, Mimura T, Tazawa M (1989) Patch-clamp study on a Ca2+-regulated K+ channel in the tonoplast of the brackish Characeae Lamprothamnium succinctum. Plant Cell Physiol 30:549–555Google Scholar
  39. Katsuhara M, Mimura T, Tazawa M (1990) ATP-regulated ion channels in the plasma membrane of a Characeae alga, Nitellopsis obtusa. Plant Physiol 93:343–346PubMedGoogle Scholar
  40. Katsuhara M, Mimura T, Tazawa M (1991) Patch-clamp study on ion channels in the tonoplast of Nitellopsis obtusa. Plant Cell Physiol 32:179–184Google Scholar
  41. Keifer DW, Spanswick RM (1979) Correlation adenosine triphosphate levels in Chara corallina with the activity of electrogenic pump. Plant Physiol 64:165–168PubMedGoogle Scholar
  42. Kersey YM, Hepler PK, Palevitz BA, Wessels NK (1976) Polarity of actin filaments in characean algae. Proc Natl Acad Sci USA 73:165–167PubMedGoogle Scholar
  43. Kikuyama M, Tazawa M (1976) Tonoplast action potential in Nitella in relation to vacuolar chloride concentration. J Membr Biol 29:95–110PubMedGoogle Scholar
  44. Kikuyama M, Tazawa M (1982) Ca2+ reversibly inhibits the cytoplasmic streaming of Nitella. Protoplasma 113:241–243Google Scholar
  45. Kikuyama M, Hayama T, Fujii S, Tazawa M (1979) Relationship between light-induced potential change and internal ATP concentration in tonoplast-free Chara cells. Plant Cell Physiol 20:993–1002Google Scholar
  46. Kikuyama M, Oda K, Shimmen T, Hayama T, Tazawa M (1984) Potassium and chloride effluxes during excitation of Characeae cells. Plant Cell Physiol 25:965–974Google Scholar
  47. Kikuyama M, Shimada K, Hiramoto Y (1993) Cessation of cytoplasmic streaming follows an increase of cytoplasmic Ca2+ during action potential in Nitella. Protoplasma 174:142–146Google Scholar
  48. Kikuyama M, Tazawa M, Tominaga Y, Shimmen T (1996) Membrane control of cytoplasmic streaming in Characeae cells. J Plant Res 109:113–118Google Scholar
  49. Kishimoto U, Tazawa M (1965a) Ionic composition of the cytoplasm of Nitella flexilis. Plant Cell Physiol 6:507–518Google Scholar
  50. Kishimoto U, Tazawa M (1965b) Ionic composition and electric response of Lamprothamnium succinctum. Plant Cell Physiol 6:529–536Google Scholar
  51. Kishimoto U, Nagai R, Tazawa M (1965) Plasmalemma potential in Nitella. Plant Cell Physiol 6:519–528Google Scholar
  52. Kiyosawa K, Tazawa M (1972) Influence of intracellular and extracellular tonicities on water permeability in Characeae cells. Protoplasma 74:257–270Google Scholar
  53. Kiyosawa K, Tazawa M (1973) Rectification characteristics of Nitella membrane in respect to water permeability. Protoplasma 78:203–214Google Scholar
  54. Kiyosawa K, Tazawa M (1977) Hydraulic conductivity of tonoplast-free Chara cells. J Membr Biol 37:157–166Google Scholar
  55. Kohama K, Kendrick-Jones J (1986) The inhibitory Ca2+–regulation of the actin-activated Mg-ATPase activity of myosin from Physarum polycephalum plasmodia. J Biochem 99:1433–1446PubMedGoogle Scholar
  56. Kohama K, Shimmen T (1985) Inhibitory Ca2+–control of movement of beads coated with Physarum myosin along actin-cables in Chara. Protoplasma 129:88–91Google Scholar
  57. Kohno T, Okagaki T, Kohama K, Shimmen T (1991) Role of actin in the myosin-linked Ca2+– regulation of ATP-dependent interaction between actin and myosin of a lower eukaryote, Physarum polycephalum. J Biochem 110:508–513PubMedGoogle Scholar
  58. Kron SJ, Spudich JA (1986) Fluorescent actin filaments move on myosin fixed to a glass surface. Proc Natl Acad Sci USA 83:6272–6276PubMedGoogle Scholar
  59. Kuroda K (1983) Cytoplasmic streaming in characean cells cut open by microsurgery. Proc Jpn Acad 59:126–130Google Scholar
  60. Kuroda K (1990) Cytoplasmic streaming in plant cells. Int Rev Cytol 121:267–307Google Scholar
  61. Kuroda K, Kamiya N (1975) Active movement of Nitella chloroplast in vitro. Proc Jpn Acad 51:774–777Google Scholar
  62. Maurel C (1997) Aquaporins and water permeability of plant membranes. Annu Rev Plant Physiol Plant Mol Biol 48:399–429PubMedGoogle Scholar
  63. McCurdy DW, Harmon AC (1992) Calcium-dependent protein kinase in the green alga Chara. Planta 188:54–61Google Scholar
  64. Mimura T, Tazawa M (1986) Light-induced membrane hyperpolarization and adenine nucleotide levels in perfused characean cells. Plant Cell Physiol 27:319–330Google Scholar
  65. Mimura T, Shimmen T, Tazawa M (1983) Dependence of the membrane potential on intracellular ATP concentration in tonoplast-free Chara cells. Planta 157:97–104Google Scholar
  66. Mimura T, Simmen T, Tazawa M (1984) Adenine-nucleotide levels and metabolism-dependent membrane potential in cells of Nitellopsis obtusa. Planta 162:77–84Google Scholar
  67. Mimura T, Sakano K, Tazawa M (1990) Changes in subcellular distribution of free amino acids in relation to light conditions in cells of Chara corallina. Bot Acta 103:42–47Google Scholar
  68. Morimatsu M, Hasegawa S, Higashi-Fujime S (2002) Protein phosphorylation regulates actomyosin-driven vesicle movement in cell extracts isolated from the green algae, Chara corallina. Cell Motil Cytoskeleton 53:66–76PubMedGoogle Scholar
  69. Moriyasu Y, Tazawa M (1986) Plant vacuole degrades exogenous proteins. Protoplasma 130:214–215Google Scholar
  70. Moriyasu Y, Tazawa M (1987) Calcium-activated protease in the giant alga Chara australis. Protoplasma 140:72–74Google Scholar
  71. Moriyasu Y, Wayne R (2004) A novel calcium-activated protease in Chara corallina. Eur J Phycol 39:57–66Google Scholar
  72. Moriyasu Y, Shimmen T, Tazawa M (1984a) Vacuolar pH regulation in Chara australis. Cell Struct Funct 9:225–234Google Scholar
  73. Moriyasu Y, Shimmen T, Tazawa M (1984b) Electric characteristics of the vacuolar membrane of Chara in relation to pHv regulation. Cell Struct Funct 9:235–246Google Scholar
  74. Nagai R, Rebhun L (1966) Cytoplasmic microfilaments in streaming Nitella cells. J Ultrastruct Res 14:571–589PubMedGoogle Scholar
  75. Nagai R, Tazawa M (1962) Changes in resting potential and ion absorption by light in a single plant cell. Plant Cell Physiol 3:323–339Google Scholar
  76. Nakagawa S, Kataoka H, Tazawa M (1974) Osmotic and ionic regulation in Nitella. Plant Cell Physiol 15:457–468Google Scholar
  77. Okazaki Y (1996) Turgor regulation in a brackish water charophyte, Lamprothamnium succinctum. J Plant Res 109:107–112Google Scholar
  78. Okazaki Y, Tazawa M (1986a) Involvement of calcium ion in turgor regulation upon hypotonic treatment in Lamprothamnium succinctum. Plant Cell Environ 9:185–190Google Scholar
  79. Okazaki Y, Tazawa M (1986b) Ca2+ antagonist nifedipine inhibits turgor regulation upon hypotonic treatment in internodal cells of Lamprothamnium. Protoplasma 134:65–66Google Scholar
  80. Okazaki Y, Tazawa M (1990) Calcium ion and turgor regulation in plant cells. J Membr Biol 114:189–194PubMedGoogle Scholar
  81. Okazaki Y, Shimmen T, Tazawa M (1984a) Turgor regulation in a brackish charophyte. Lamprothamnium succinctum. I. Artificial modification of intracellular osmotic pressure. Plant Cell Physiol 25:565–571Google Scholar
  82. Okazaki Y, Shimmen T, Tazawa M (1984b) Turgor regulation in a brackish charophyte, Lamprothamnium succinctum. II. Changes in K+, Na+ and Cl concentrations, membrane potential and membrane resistance during turgor regulation. Plant Cell Physiol 25:573–581Google Scholar
  83. Okazaki Y, Yoshimoto Y, Hiramoto Y, Tazawa M (1987) Turgor regulation and cytoplasmic free Ca2+ in the alga Lamprothamnium. Protoplasma 140:67–71Google Scholar
  84. Okazaki Y, Tazawa M, Moriyama Y, Iwasaki N (1992) Bafilomycin inhibits vacuolar pH regulation in a fresh water charophyte, Chara corallina. Bot Acta 105:421–426Google Scholar
  85. Sakamura T (1952) Physiology of osmosis in plant cells (in Japanese). Yokendo, TokyoGoogle Scholar
  86. Sakano K, Tazawa M (1985) Metabolic conversion of amino acids loaded in the vacuole of Chara australis internodal cells. Plant Physiol 78:673–677PubMedGoogle Scholar
  87. Sakano K, Tazawa M (1986) Tonoplast origin of the envelope membrane of cytoplasmic droplets prepared from Chara internodal cells. Protoplasma 131:247–249Google Scholar
  88. Sheez MP, Spudich JA (1983) Myosin-coated fluorescent beads move on actin cables in vitro. Nature 303:31–35Google Scholar
  89. Shiina T, Tazawa M (1987a) Demonstration and characterization of Ca2+ channel in tonoplast-free cells of Nitellopsis obtusa. J Membr Biol 96:263–276Google Scholar
  90. Shiina T, Tazawa M (1987b) Ca2+-activated Cl channel in plasmalemma of Nitellopsis obtusa. J Membr Biol 99:137–146Google Scholar
  91. Shiina T, Tazawa M (1988) Ca2+-dependent Cl efflux in tonoplast-free cells of Nitellopsis obtusa. J Membr Biol 106:135–139Google Scholar
  92. Shiina T, Wayne R, Lim Tung HY, Tazawa M (1988) Possible involvement of protein phosphorylation/dephosphorylation in the modulation of Ca2+ channel in tonoplast-free cells of Nitellopsis. J Membr Biol 102:255–264Google Scholar
  93. Shimmen T (2003) Studies on mechano-perception in the Characeae: transduction of pressure signals into electrical signals. Plant Cell Physiol 44:1215–1224PubMedGoogle Scholar
  94. Shimmen T (2006a) Electrical perception of “death message” in Chara: characterization of K+-induced depolarization. Plant Cell Physiol 47:559–562PubMedGoogle Scholar
  95. Shimmen T (2006b) Electrophysiology in mechanosensing and wounding response. In: Volkov AG (ed) Plant electrophysiology – theory and methods. Springer, Berlin, pp 319–339Google Scholar
  96. Shimmen T (2007) The sliding theory of cytoplasmic streaming: fifty years of progress. J Plant Res 120:31–43PubMedGoogle Scholar
  97. Shimmen T (2008) Electrophysiological characterization of the node in Chara corallina: functional differentiation for wounding response. Plant Cell Physiol 49:264–272PubMedGoogle Scholar
  98. Shimmen T, Nishikawa S (1988) Studies on the tonoplast action potential of Nitella flexilis. J Membr Biol 101:133–140Google Scholar
  99. Shimmen T, Tazawa M (1977) Control of membrane potential and excitability of Chara cells with ATP and Mg2+. J Membr Biol 37:167–192Google Scholar
  100. Shimmen T, Tazawa M (1980) Intracellular chloride and potassium ions in relation to excitability of Chara membrane. J Membr Biol 55:223–232Google Scholar
  101. Shimmen T, Tazawa M (1982a) Cytoplasmic streaming in the cell model of Nitella. Protoplasma 113:127–131Google Scholar
  102. Shimmen T, Tazawa M (1982b) Reconstitution of cytoplasmic streaming in Characeae. Protoplasma 113:127–131Google Scholar
  103. Shimmen T, Tazawa M (1982c) Effects of intracellular vanadate on electrogenesis, excitability and cytoplasmic streaming in Nitellopsis obtusa. Plant Cell Physiol 23:669–677Google Scholar
  104. Shimmen T, Yano M (1984) Active sliding movement of latex beads coatred with skeletal muscle myosin on Chara actin bundles. Protoplasma 121:132–137Google Scholar
  105. Shimmen T, Kikuyama M, Tazawa M (1976) Demonstration of two stable potential states of plasmalemma of Chara without tonoplast. J Membr Biol 30:225–247Google Scholar
  106. Staves MP, Wayne R, Leopold AC (1992) Hydrostatic pressure mimics gravitational pressure in characean cells. Protoplasma 168:141–152PubMedGoogle Scholar
  107. Takeshige K, Shimmen T, Tazawa M (1985) Electrogenic pump current and ATP-dependent H+ efflux across the plasma membrane of Nitellopsis obtusa. Plant Cell Physiol 26:661–668Google Scholar
  108. Takeshige K, Tazawa M, Hager A (1988) Characterization of the H+ translocating adenosine triphosphatase and pyrophosphatase of vacuolar membranes isolated by means of a perfusion technique from Chara corallina. Plant Physiol 86:1168–1173PubMedGoogle Scholar
  109. Taylor DL, Condeelis JS, Moore PL, Allen RD (1973) The contractile basis of amoeboid movement. The chemical control of motility in isolated cytoplasm. J Cell Biol 59:378–394PubMedGoogle Scholar
  110. Tazawa M (1964) Studies on Nitella having artificial cell sap. I. Replacement of the cell sap with artificial solutions. Plant Cell Physiol 5:33–43Google Scholar
  111. Tazawa M (1968) Motive force of the cytoplasmic streaming in Nitella. Protoplasma 65:207–222PubMedGoogle Scholar
  112. Tazawa M, Kamiya N (1965) Water permeability of a characean internodal cell. Ann Rep Biol Works Fac Sci Osaka Univ 13:123–157Google Scholar
  113. Tazawa M, Kamiya N (1966) Water relations of characean internodal cell with special reference to its polarity. Aust J Biol Sci 19:399–419Google Scholar
  114. Tazawa M, Kishimoto U (1968) Cessation of cytoplasmic streaming of Chara internodes during action potential. Plant Cell Physiol 9:361–368Google Scholar
  115. Tazawa M, Kiyosawa K (1973) Analysis of transcellular water movement in Nitella. A new procedure to determine the inward and outward water permeabilities of membranes. Protoplasma 78:349–364Google Scholar
  116. Tazawa M, Nagai R (1960) Die Mitwirkung von Ionen bei der Osmoregulation der Nitellazelle. Plant Cell Physiol 1:255–267Google Scholar
  117. Tazawa M, Nagai R (1966) Studies on osmoregulation of Nitella internode with modified cell saps. Z Pflanzenphysiol 54:333–344Google Scholar
  118. Tazawa M, Kikuyama M, Nakagawa S (1975) Open-vacuole method for measuring membrane potential and membrane resistance of Characeae cells. Plant Cell Physiol 16:611–621Google Scholar
  119. Tazawa M, Kikuyama M, Shimmen T (1976) Electric characteristics and cytoplasmic streaming of Characeae cells lacking tonoplast. Cell Struct Funct 1:165–176Google Scholar
  120. Tazawa M, Shimmen T, Mimura T (1987) Membrane control in the Characeae. Ann Rev Plant Physiol 38:95–117Google Scholar
  121. Tazawa M, Okazaki Y, Moriyama Y, Iwasaki N (1995) Concanamycin 4-B: a potent inhibitor of vacuolar pH regulation in Chara cells. Bot Acta 108:67–73Google Scholar
  122. Tominaga Y, Shimmen T, Tazawa M (1983) Control of cytoplasmic streaming by extracellular Ca2+ in permeabilized Nitella cells. Protoplasma 116:75–77Google Scholar
  123. Tominaga Y, Muto S, Shimmen T, Tazawa M (1985) Calmodulin and Ca2+-controlled cytoplasmic streaming in characean cells. Cell Struct Funct 10:315–325Google Scholar
  124. Tominaga Y, Wayne R, Tung HYL, Tazawa M (1987) Phosphorylation-dephosphorylation is involved in Ca2+-controlled cytoplasmic streaming of characean cells. Protoplasma 136:161–169Google Scholar
  125. Ward JM, Mäser P, Schroeder JI (2009) Plant ion channels: gene families, physiology, and functional genomics analyses. Annu Rev Physiol 71:59–82PubMedGoogle Scholar
  126. Wayne R (2009) Plant cell biology: from astronomy to zoology. Elsevier, AmsterdamGoogle Scholar
  127. Wayne R, Tazawa M (1990) Nature of the water channels in the internodal cells of Nitellopsis. J Membr Biol 116:31–39PubMedGoogle Scholar
  128. Williamson RE (1974) Actin in the alga, Chara corallina. Nature 248:801–802PubMedGoogle Scholar
  129. Williamson RE (1975) Cytoplasmic streaming in Chara: a cell model activated by ATP and inhibited by cytochalasin B. J Cell Sci 17:655–668PubMedGoogle Scholar
  130. Williamson RE, Ashley CC (1982) Free Ca2+ and cytoplasmic streaming in the alga Chara. Nature 296:647–651PubMedGoogle Scholar
  131. Yamamoto K, Kikuyama M, Sutoh-Yamamoto N, Kamitsubo E (1994) Purification of actin based motor protein from Chara corallina. Proc Jpn Acad 70:175–180Google Scholar
  132. Yangida T, Nakase M, Nishiyama K, Oosawa F (1984) Direct observation of single actin filaments in the presence of myosin. Nature 307:58–60Google Scholar
  133. Ye Q, Wiera B, Steudle E (2004) A cohesioin/tension mechanism explains the gating of water channels (aquaporins) in Chara internodes by high concentration. J Exp Bot 55:449–461PubMedGoogle Scholar
  134. Yokota E, Shimmen T (1994) Isolation and characterization of plant myosin from pollen tubes of lily. Protoplasma 177:153–162Google Scholar
  135. Yuasa T, Okazaki Y, Iwasaki N, Muto S (1997) Involvement of a calcium-dependent protein kinase in hypoosmotic turgor regulation in a brackish water Characeae Lamprothamnium succinctum. Plant Cell Physiol 38:586–594Google Scholar

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Authors and Affiliations

  1. 1.The University of TokyoOtsuJapan

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