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New Trends in Fungal Biooxidation

Chapter
Part of the The Mycota book series (MYCOTA, volume 10)

Abstract

Biooxidations using isolated biocatalysts for the oxidative conversion of organic compounds and materials are becoming more and more important in the industrial sector. Research in this direction is part of the rapidly developing field of White Biotechnology and is carried out not least against the background of sustainable development and the need to replace environmentally risky technologies by eco-friendly processes. Whereas whole cells are already widely used as oxidative biocatalysts, the application of isolated enzymes is still limited to a few examples. One reason for that is the cost-intensive production and laborious purification of enzymes which are mostly intracellular and sometimes membrane-bound proteins with low stability and complex co-factor requirements. The use of secreted oxidoreductases offers several advantages: they are easier to separate and purify, need only cheap co-substrates such as dioxygen or peroxides and are far more stable than intracellular enzymes. Filamentous fungi secrete a broad spectrum of oxidative biocatalysts which are involved, amongst others, in the degradation of recalcitrant biopolymers, the synthesis of melanins as well as the detoxification of plant ingredients, microbial metabolites and organopollutants. We focus here on novel secreted enzymes - peroxygenases and DyP-type peroxidases - with remarkable catalytic properties. Furthermore selected features of “classic” fungal oxidoreductases, such as laccase, tyrosinase and chloroperoxidase, are discussed against the background of innovative recent developments in the field of enzyme application.

Keywords

Biofuel Cell Heme Peroxidase Pycnoporus Cinnabarinus Myceliophthora Thermophila Agrocybe Aegerita 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We would like to thank M. Pecyna, M. Kinne, M. Inge Kluge, S. Peter, C. Liers, K. Barková, M. Poraj-Kobielska and G. Gröbe for still unpublished results on aromatic peroxygenases. The work in our laboratories was supported by the European Union (integrated project Biorenew), the Deutsche Bundestiftung Umwelt (DBU; projects Pilzliche Peroxygenasen and Pilzliche Sekretome), the Bundesministerium für Forschung (BMBF) and the Deutsche Forschungsgemeinschaft (DFG; projects FUPERS and FUNWOOD).

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© Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  1. 1.Department of Bio- and Environmental SciencesInternational Graduate School of ZittauZittauGermany

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