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Xenon Biosensors for Multi-Purpose Molecular Imaging

  • Leif Schröder
  • Tyler Meldrum
  • Monica Smith
  • Franz Schilling
  • Philipp Denger
  • Sina Zapf
  • David Wemmer
  • Alexander Pines
Conference paper
Part of the IFMBE Proceedings book series (IFMBE, volume 25/13)

Abstract

Hyperpolarized xenon is an exquisite NMR probe for sensing molecular environments of the noble gas in solution. By trapping it in molecular cages like cryptophane-A, 129Xe can report information about molecular-specific binding events or resolve multiple signals simultaneously from different micro-environments in a lipid emulsion-a macroscopically-homogeneous phase that mimics properties of biological relevance. The Hyper-CEST detection scheme can be used in this context to pair significant signal enhancement with high specificity of xenon NMR resonances. Hyper-CEST can reduce the measurement time by a factor of up to 16 million and is currently able to detect biosensor concentrations as low as 1.4 nM. When combined with highly frequency-selective pulses, it also allows for demonstration of multiplexing potential using a single cage type as contrast agent for different environments in NMR imaging. This molecular imaging approach enables a switchable contrast that includes also temperature-sensitive imaging with molecular sensors that can be functionalized with various targeting molecules to bind, e.g., specifically to receptors of cancer cells.

Keywords

Biosensors Hyper-CEST xenon hyperpolarization molecular imaging 

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Copyright information

© International Federation for Medical and Biological Engineering 2009

Authors and Affiliations

  • Leif Schröder
    • 1
    • 2
  • Tyler Meldrum
    • 1
    • 3
  • Monica Smith
    • 1
    • 3
  • Franz Schilling
    • 1
    • 4
  • Philipp Denger
    • 5
  • Sina Zapf
    • 4
  • David Wemmer
    • 1
    • 3
  • Alexander Pines
    • 1
    • 3
  1. 1.Lawrence Berkeley National Lab, Materials Sciences and Physical Biosciences DivisionsBerkeleyUSA
  2. 2.Leibniz-Institut für Molekulare PharmakologieBerlinGermany
  3. 3.Department of Chemistry and qb3 Institute for Quantitative BiosciencesUniversity of California at BerkeleyBerkeleyUSA
  4. 4.Julius-Maximilians-Universität Würzburg, Experimentelle Physik VWürzburgGermany
  5. 5.Deutsches Krebsforschungszentrum, Abteilung Medizinische Physik in der RadiologieHeidelbergGermany

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