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Insights into Function of the Immunological Synapse from Studies with Supported Planar Bilayers

  • Michael L. Dustin
Chapter
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 340)

Abstract

Innate and adaptive immunity is dependent upon reliable cell–cell communication mediated by direct interactions of cell surface receptors with ligands integrated into the surface of apposing cells or bound directly to the surface as in complement deposition or antibody mediated recognition through Fc receptors. Supported lipid bilayers formed on glass surfaces offer a useful model system in which to explore some basic features of molecular interactions in immunological relevant contacts, which include signal integration and effector functions through immunological synapses and kinapses. We have exploited that lateral mobility of molecules in the supported planar bilayers and fluorescence microscopy to develop a system for measurement of two-dimensional affinities and kinetic rates in the contact area, which is of immunological interest. Affinity measurements are based on a modified Scatchard analysis. Measurements of kinetic rates are based on fluorescence photo bleaching after recovery at the level of the entire contact area. This has been coupled to a reaction–diffusion equation that allows calculation of on- and off-rates. We have found that mixtures of ligands in supported planar bilayers can effectively activate T lymphocytes and simultaneously allow monitoring of the immunological synapse. Recent studies in planar bilayers have provided additional insights into organization principles of cell–cell interfaces. Perennial problems in understanding cell–cell communication are yielding quantitative measurements based on planar bilayers in areas of ligand-driven receptor clustering and the role of the actin cytoskeleton in immune cell activation. A major goal for the field is determining quantitative rules involved in signaling complex formation by innate and adaptive receptor systems.

Keywords

Affinity Antibodies Avidity Bilayer Cytoskeleton Kinapse Receptors Signaling Synapse 

Abbreviations

BCR

B-cell antigen receptor

LFA

Lymphocyte function associated

PKC

Protein kinase C

SMAC

Supramolecular activation cluster

TCR

T-cell antigen receptor

TIRF

Total internal reflection fluorescence

Notes

Acknowledgments

I thank the members of my lab who have contributed to many of the studies highlighted in this commentary. I thank Mike Sheetz for discussion of implications of bead size threshold for TCR stimulation.

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© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Helen L. and Martin S. Kimmel Center for Biology and Medicine in the Skirball Institute for Biomolecular Medicine and Department of PathologyNYU School of MedicineNew YorkUSA

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