Abstract
Colorectal carcinoma is the second most common malignancy among men and women worldwide. Molecular markers that enable to identify metastatic processes are needed urgently. An early identification of patients with metastases or with a high probability that the primary tumor will spread to distant sites could influence the aggressiveness of the therapy, e.g. adjuvant chemotherapy.
In this study five non-metastasized (UICC I and II) and four metastasized (UICC III) specimens from the Department of Surgery, as well as the corresponding normal mucosa from these patients, were included.
A pairwise comparison of the groups metastasized versus non-metastasized was done with differential 2D In-Gel-Electrophoresis (DIGE). A high reproductivity of the DIGE-Methodwas achieved. First analyses revealed two major differentially expressed proteins between the two groups. These proteins were identified via MALDI-TOF-MS as Transgelin and HLA-A2, which have been described previously to be regulated in the colorectal carcinoma. Further analyses are subject of ongoing studies. Verification of the results will be performed using Western Blot and immunohisto-chemistry.
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© 2009 Springer Medizin Verlag Heidelberg
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Sauermann, A.C. et al. (2009). Comparative proteomics-analysis for the isolation of markers for metastasis of the colorectal carcinoma. In: Schumpelick, V., Bruch, H.P., Schackert, H.K. (eds) Chirurgisches Forum und DGAV Forum 2009. Deutsche Gesellschaft für Chirurgie, vol 38. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-00625-8_89
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DOI: https://doi.org/10.1007/978-3-642-00625-8_89
Publisher Name: Springer, Berlin, Heidelberg
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