Microdevice for Trapping Circulating Tumor Cells for Cancer Diagnostics
Cancer metastasis is the main attribute to cancerrelated deaths and early detection is one of the most effective means to treat the disease. Spreading of cancer to distant sites is achieved usually through the circulatory system and the number of circulating tumor cells (CTCs) in peripheral blood is strongly associated to cancer development. Thus detecting CTCs in blood samples will be helpful in identifying susceptible subjects. Here, we propose a label-free technique to isolate these cancerous cells from blood. Using cancer cells spiked in blood samples, isolation efficiency of at least 80% were obtained with high isolation purity. The microdevice also preserved the integrity of the isolated cells to allow further studies to be done on these cells. These results showed a potential effective microdevice for CTCs studies and possible clinical application.
KeywordsCell Isolation Microfluidics Circulating Tumor Cells Cancer Metastasis Computational Fluid Dynamics (CFD)
Unable to display preview. Download preview PDF.
- 7.Balic M, Dandachi N, Hofmann G, et al. (2005) Comparison of two methods for enumerating circulating tumor cells in carcinoma patients. Cytometry B Clin Cytom 68:25–30Google Scholar
- 11.Shelby J P, White J, Ganesan K, et al. (2003) A microfluidic model for single-cell capillary obstruction by Plasmodium falciparuminfected erythrocytes. 100:14618–14622Google Scholar
- 15.Tan S J, Yobas L, Lee G Y H, et al. (2008) Microdevice for Isolating Viable Circulating Tumor Cells, International Conference on Biocomputation, Bioinformatics, and Biomedical Technologies, 2008. BIOTECHNO’ 08., Bucharest, Romania, 2008, pp. 109–113Google Scholar
- 18.Dawood S, Broglio K, Valero V, et al. (2008) Circulating tumor cells in metastatic breast cancer: from prognostic stratification to modification of the staging system? Cancer Google Scholar