Calibration in fluorescence correlation spectroscopy for measurements of stem cell differentiation kinetic

  • D. Dumas
  • B. Riquelme
  • H. Castellini
  • L. Basciano
  • N. de Isla
  • J F Stoltz
Conference paper

Abstract

Several works have recently shown that Mesenchymal Stem Cells (MSC) collected from bone marrow can differentiate in vitro into cartilage cells under the effects of transforming growth factors or critical transcription factors [1]. The transforming growth factor TGF-β is a family of multifunctional cytokines controlling cell growth, differentiation, and extra cellular matrix deposition. The biological effects of TGF-β are mediated by type I (TBR-I) and II (TBR-II) receptors. In this work we presented a preliminary study to characterize the localization and mobility of TBR-II using mesenchymal stem cells derived from bone marrow by Fluorescence Correlation Spectroscopy (FCS). This sensitive technique is mainly used for biological applications for measuring dynamic processes (number density, interaction fractions and molecular dynamics) in a fluorescent signal on the nanosecond to second time scales [2]. To apply this analytical method to living cells, some steps of standardization have to be optimized in the case of indirect immunolabeling.

Keywords

mesenchymal stem cells fluorescence correlation spectroscopy cell differentiation 
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References

  1. 1.
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    D Dumas, B Riquelme, E. Werkmeister, N. de Isla, JF Stoltz. Multimodality of Microscopy Imaging Applied to Cartilage Tissue Engineering. In. Ostearthitis, Inflamation and Degradation: A Continum, Capitulo XVI, J. Buckwalter et al. (Eds.), IOS Press, 2007, pp. 254–260Google Scholar
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    S. Hess and W. Webb. “Focal Volume Optics and Experimental Artifacts in Confocal Fluorescence Correlation Spectroscopy”. Biophysical Journal Volume 83 October 2002 2300–2317.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2008

Authors and Affiliations

  • D. Dumas
    • 2
  • B. Riquelme
    • 1
  • H. Castellini
    • 3
  • L. Basciano
    • 1
    • 2
  • N. de Isla
    • 2
  • J F Stoltz
    • 2
  1. 1.Physics Area at the Faculty of Biochemical and Pharmacology Sciences and Biology Applied Optics Groups at the Rosario Physical InstituteCONICET-UNRArgentina
  2. 2.Lab. Mecanique et Ingenierie Cellulaire et Tissulaire — UMR CNRS 7563 LEMTA et IFR 111 CNRSUniversité Henry PoincaréNancyFrance
  3. 3.Dpto. Física, Fac. Cs Exactas Ing. y AgrUNRosarioArgentina

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