Visualization of detergent resistant membrane rafts in human colorectal cancer cells with correlative confocal and transmission electron microscopy
Detergent resistant membrane domains (DRM) can for the first time be visualised in the context of their native environment (i.e. attached to a cell) using correlative fluorescence and electron microscopy (CFEM). DRMs found on whole mounted colorectal cancer cells have a size range that is consistent with that widely accepted for lipid rafts (50–100nm). However, micron sized domains have also been observed. Furthermore, CFEM provides a tool to study DRMs on living cells yet still allows them to be prepared for high-resolution transmission electron microscopy (TEM) and electron tomographic analysis.
KeywordsDRM correlative microscopy actin cytoskeleton
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