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Visualization of detergent resistant membrane rafts in human colorectal cancer cells with correlative confocal and transmission electron microscopy

  • K. Jahn
  • E. P. Kable
  • F. Braet
Conference paper

Abstract

Detergent resistant membrane domains (DRM) can for the first time be visualised in the context of their native environment (i.e. attached to a cell) using correlative fluorescence and electron microscopy (CFEM). DRMs found on whole mounted colorectal cancer cells have a size range that is consistent with that widely accepted for lipid rafts (50–100nm). However, micron sized domains have also been observed. Furthermore, CFEM provides a tool to study DRMs on living cells yet still allows them to be prepared for high-resolution transmission electron microscopy (TEM) and electron tomographic analysis.

Keywords

DRM correlative microscopy actin cytoskeleton 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2008

Authors and Affiliations

  • K. Jahn
    • 1
  • E. P. Kable
    • 1
  • F. Braet
    • 1
  1. 1.Australian Key Centre for Microscopy and MicroanalysisThe University of SydneyAustralia

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