Visualization of detergent resistant membrane rafts in human colorectal cancer cells with correlative confocal and transmission electron microscopy

  • K. Jahn
  • E. P. Kable
  • F. Braet
Conference paper


Detergent resistant membrane domains (DRM) can for the first time be visualised in the context of their native environment (i.e. attached to a cell) using correlative fluorescence and electron microscopy (CFEM). DRMs found on whole mounted colorectal cancer cells have a size range that is consistent with that widely accepted for lipid rafts (50–100nm). However, micron sized domains have also been observed. Furthermore, CFEM provides a tool to study DRMs on living cells yet still allows them to be prepared for high-resolution transmission electron microscopy (TEM) and electron tomographic analysis.


DRM correlative microscopy actin cytoskeleton 


  1. 1.
    V. Michel and M. Bakovic, Biol Cell 99 (2007) 129.CrossRefGoogle Scholar
  2. 2.
    K. Simons and E. Ikonen, Nature 387 (1997) 569.CrossRefADSGoogle Scholar
  3. 3.
    M. Edidin, Annu Rev Biophys Biomol Struct 32 (2003) 257.CrossRefGoogle Scholar
  4. 4.
    D. A. Brown and J. K. Rose, Cell 68 (1992) 533.CrossRefGoogle Scholar
  5. 5.
    B. C. Lagerholm, G. E. Weinreb, K. Jacobson et al., Annu Rev Phys Chem 56 (2005) 309.CrossRefADSGoogle Scholar
  6. 6.
    K. A. Jahn, D. Barton, and F. Braet, Méndez-Vilas A, Labajos-Broncano L, eds. Current Issues on Multidisciplinary Microscopy Research and Education III (2007)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2008

Authors and Affiliations

  • K. Jahn
    • 1
  • E. P. Kable
    • 1
  • F. Braet
    • 1
  1. 1.Australian Key Centre for Microscopy and MicroanalysisThe University of SydneyAustralia

Personalised recommendations