Micro and Nano Patterning for Cell and Tissue Engineering

  • Shyam Patel
  • Hayley Lam
  • Song Li


One of the goals of bioengineers today is to answer the basic question of how individual cells, which together comprise an entire living being, communicate and interact with one another and the physical and chemical environment in which they reside. In this chapter, we will focus on the micro and nano technologies that have been used to investigate the regulation of cell functions by micro and nano features in matrix distribution, surface topography and three-dimensional (3D) microenvironment, and explore the technologies that can be applied to the fabrication of functional tissue constructs.


Tissue Engineer Neurite Extension Matrix Patterning Microcontact Printing Polymer Mold 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Cao Y, Vacanti JP, Paige KT, Upton J, Vacanti CA (1997) Plast Reconstr Surg 100:297–302; discussion 303–304CrossRefGoogle Scholar
  2. 2.
    Singhvi R, Kumar A, Lopez GP, Stephanopoulos GN, Wang DI, Whitesides GM, Ingber DE (1994) Science 264:696–698CrossRefGoogle Scholar
  3. 3.
    Li Jeon N, Baskaran H, Dertinger SK, Whitesides GM, Van de Water L, Toner M (2002) Nat Biotechnol 20:826–830Google Scholar
  4. 4.
    Chen CS, Mrksich M, Huang S, Whitesides GM, Ingber DE (1997) Science 276:1425–1428CrossRefGoogle Scholar
  5. 5.
    Thakar RG, Ho F, Huang NF, Liepmann D, Li S (2003) Biochem Biophys Res Commun 307:883–890CrossRefGoogle Scholar
  6. 6.
    Jiang X, Bruzewicz DA, Wong AP, Piel M, Whitesides GM (2005) Proc Natl Acad Sci USA 102:975–978CrossRefGoogle Scholar
  7. 7.
    Li S, Bhatia S, Hu YL, Shiu YT, Li YS, Usami S, Chien S (2001) Biorheology 38:101–108Google Scholar
  8. 8.
    Hsu S, Thakar R, Liepmann D, Li S (2005) Biochem Biophys Res Commun 337:401–409CrossRefGoogle Scholar
  9. 9.
    Dertinger SK, Jiang X, Li Z, Murthy VN, Whitesides GM (2002) Proc Natl Acad Sci USA 99:12542–12547CrossRefGoogle Scholar
  10. 10.
    Clark P, Britland S, Connolly P (1993) J Cell Sci 105(1):203–212Google Scholar
  11. 11.
    McBeath R, Pirone DM, Nelson CM, Bhadriraju K, Chen CS (2004) Dev Cell 6:483–495CrossRefGoogle Scholar
  12. 12.
    Flaim CJ, Chien S, Bhatia SN (2005) Nat Methods 2:119–125CrossRefGoogle Scholar
  13. 13.
    Huang NF, Patel S, Thakar RG, Wu J, Hsiao BS, Chu B, Lee RJ, Li S (2006) Nano Lett 6:537–542CrossRefGoogle Scholar
  14. 14.
    Turner DC, Lawton J, Dollenmeier P, Ehrismann R, Chiquet M (1983) Dev Biol 95:497–504CrossRefGoogle Scholar
  15. 15.
    Nakatsuji N, Johnson KE (1984) Nature 307:453–455CrossRefGoogle Scholar
  16. 16.
    Curtis A, Wilkinson C (1999) Biochem Soc Symp 65:15–26Google Scholar
  17. 17.
    Balaban NQ, Schwarz US, Riveline D, Goichberg P, Tzur G, Sabanay I, Mahalu D, Safran S, Bershadsky A, Addadi L, Geiger B (2001) in Nat Cell Biol 3:466–472CrossRefGoogle Scholar
  18. 18.
    Tan JL, Tien J, Pirone DM, Gray DS, Bhadriraju K, Chen CS (2003) Proc Natl Acad Sci USA 100:1484–1489CrossRefGoogle Scholar
  19. 19.
    Park A, Wu B, Griffith LG (1998) J Biomater Sci Polym Ed 9:89–110CrossRefGoogle Scholar
  20. 20.
    Albrecht DR, Underhill GH, Wassermann TB, Sah RL, Bhatia SN (2006) Nat Methods 3:369–375CrossRefGoogle Scholar
  21. 21.
    Tan W, Desai TA (2003) Tissue Eng 9:255–267CrossRefGoogle Scholar
  22. 22.
    Nelson CM, Vanduijn MM, Inman JL, Fletcher DA, Bissell MJ (2006) Science 314:298–300CrossRefGoogle Scholar
  23. 23.
    Li D, Ouyang G, McCann JT, Xia Y (2005) Nano Lett 5:913–916CrossRefGoogle Scholar
  24. 24.
    Yang F, Murugan R, Wang S, Ramakrishna S (2005) Biomaterials 26:2603–2610CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2008

Authors and Affiliations

  • Shyam Patel
    • 1
  • Hayley Lam
    • 1
  • Song Li
    • 1
  1. 1.Department of BioengineeringUniversity of CaliforniaBerkeleyUSA

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