RNA Interference pp 225-256

Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 320)

RNA Silencing in Mammalian Oocytes and Early Embryos

  • Petr Svoboda

Abstract

RNA silencing is a common term for homology-dependent silencing phenomena found in the majority of eukaryotic species. RNA silencing pathways share several conserved components. The common denominator of these pathways is the presence of specific, short (21–25 nt) RNA molecules generated from different double-stranded RNA substrates by a specific RNase III activity. Short RNA molecules serve as a template for sequence-specific effects including transcriptional silencing, mRNA degradation, and inhibition of translation. This review will discuss possible roles of RNA silencing pathways in mouse oocytes and early embryos as well as the use of RNA silencing for experimental inhibition of gene expression in this model system.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2008

Authors and Affiliations

  • Petr Svoboda
    • 1
  1. 1.Institute of Molecular GeneticsCzech Academy of SciencesPragueCzech Republic

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