Seed-Based Exclusion Method for Non-coding RNA Gene Search

  • Jean-Eudes Duchesne
  • Mathieu Giraud
  • Nadia El-Mabrouk
Part of the Lecture Notes in Computer Science book series (LNCS, volume 4598)

Abstract

Given an RNA family characterized by conserved sequences and folding constraints, the problem is to search for all the instances of the RNA family in a genomic database. As seed-based heuristics have been proved very efficient to accelerate the classical homology based search methods such as BLAST, we use a similar idea for RNA structures. We present an exclusion method for RNA search allowing for possible nucleotide insertion, deletion and substitution. It is based on a partition of the RNA stem-loops into consecutive seeds and a preprocessing of the target database. This algorithm can be used to improve time efficiency of current methods, and is guaranteed to find all occurrences that contain at least one exact seed.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2007

Authors and Affiliations

  • Jean-Eudes Duchesne
    • 1
  • Mathieu Giraud
    • 2
  • Nadia El-Mabrouk
    • 1
  1. 1.DIRO – Université de Montréal – H3C 3J7Canada
  2. 2.Bioinfo/Sequoia – LIFL/CNRS, Université de Lille 1France

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