Mesenchymal Stem Cells: a Modern Approach to Treat Long Bones Defects

  • Hana Krečič Stres
  • M. Krkovič
  • J. Koder
  • E. Maličev
  • D. Marolt
  • M. Drobnic
  • N. Kregar-Velikonja
Conference paper
Part of the IFMBE Proceedings book series (IFMBE, volume 16)


Human bone marrow contains a population of bone marrow stromal cells (BMSC) capable of forming several types of mesenchymal tissues, including bone and cartilage. BMSC can be isolated, purified and expanded in cell cultures in order to be subsequently implanted in vivo to facilitate bone healing. Our study was designed to a) develop autologous bone tissue constructs ex vivo – by seeding BMSC derived osteoblasts on calcium-triphosphate scaffolds, b) to apply these constructs in patients with a defect of a long bone, and c) to evaluate the healing process. Twenty patients are planed to be involved in the present clinical trial in which the efficiency of psevdoartrosis treatment by tissue engineered bone grafts will be evaluated. One patient was treated according to the study protocol, and the first results are encouraging.


Mesenchymal Stem Cell Bone Defect Osteogenic Differentiation Intramedullary Nail Autologous Bone Marrow 
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Copyright information

© Springer-Verlag Berlin Heidelberg 2007

Authors and Affiliations

  • Hana Krečič Stres
    • 1
  • M. Krkovič
    • 2
  • J. Koder
    • 3
  • E. Maličev
    • 4
  • D. Marolt
    • 2
    • 5
  • M. Drobnic
    • 6
  • N. Kregar-Velikonja
    • 1
  1. 1.Educell d.o.o.LjubljanaSlovenia
  2. 2.Department for TraumatologyUniversity Medical Centre LjubljanaLjubljanaSlovenia
  3. 3.Clinical Institute for RadiologyUniversity Medical Centre LjubljanaLjubljanaSlovenia
  4. 4.Blood Transfusion Centre of SloveniaLjubljanaSlovenia
  5. 5.Department of Biomedical EngineeringColumbia UniversityNew YorkUSA
  6. 6.Department of Orthopaedic SurgeryUniversity Medical CentreLjubljanaSlovenia

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