Mesenchymal Stem Cells: a Modern Approach to Treat Long Bones Defects
Human bone marrow contains a population of bone marrow stromal cells (BMSC) capable of forming several types of mesenchymal tissues, including bone and cartilage. BMSC can be isolated, purified and expanded in cell cultures in order to be subsequently implanted in vivo to facilitate bone healing. Our study was designed to a) develop autologous bone tissue constructs ex vivo – by seeding BMSC derived osteoblasts on calcium-triphosphate scaffolds, b) to apply these constructs in patients with a defect of a long bone, and c) to evaluate the healing process. Twenty patients are planed to be involved in the present clinical trial in which the efficiency of psevdoartrosis treatment by tissue engineered bone grafts will be evaluated. One patient was treated according to the study protocol, and the first results are encouraging.
KeywordsMesenchymal Stem Cell Bone Defect Osteogenic Differentiation Intramedullary Nail Autologous Bone Marrow
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- 2.Flanagan N (2001) Advances in stem cell therapy. Genetic Engineering News 21(9):1,29,61,66Google Scholar
- 5.Heller L, Scot LL (2001) Bone and soft tissue reconstruction. In: Bucholz RW, Heckmann JD Fractures in Adults. Lippincot Williams & Wilkins, New YorkGoogle Scholar
- 8.Martin I, Muraglia A, Campanile G, Cancedda, Quarto R (2006) Fibroblast growth factor-2 supports ex vivo expansion and maintenance of osteogenic precursors from human bone marrow. Endo 138(10):4456–4462Google Scholar
- 10.Carter DR, Beaupre GS, Giori NJ, Helms JA (1998) Mechanobiology of Skeletal Regeneration. Clin Orthop Rel Res, S41–55Google Scholar
- 13.Orozco L, Rodriguez L, Torrico C, Douville J, Hock JM, Armstrong RD et al. (2005) Clinical feasibility study: The use of cultured enriched autologous bone marrow cells to treat refractory atrophic and hypotrophic nonunion fractures at http://scholar.google.com/scholar?hl=en&lr=&q=cache:ss358GFA5X cJ:www.aastrom.com/pdf/Whitepaper_Barcelona051205. pdf+Orozco+clinical+feasibilityGoogle Scholar
- 14.Hernandez Alfaro (2005) Clinical feasibility study: The use of cultured autologous bone marrow – derived Tissue repair cells (TRC) for maxillary sinus floor au gmentation in edetulous humans at http://www.aastrom.com/corporate/bone.cfm?pagesect=SinusLiftGoogle Scholar