G-Protein-Coupled Receptor-Signaling Components in Membrane Raft and Caveolae Microdomains
The efficiency of signal transduction in cells derives in part from subcellular, in particular plasma membrane, microdomains that organize signaling molecules and signaling complexes. Two related plasma membrane domains that compartmentalize G-protein coupled receptor (GPCR) signaling complexes are lipid (membrane) rafts, domains that are enriched in certain lipids, including cholesterol and sphingolipids, and caveolae, a subset of lipid rafts that are enriched in the protein caveolin. This review focuses on the properties of lipid rafts and caveolae, the mechanisms by which they localize signaling molecules and the identity of GPCR signaling components that are organized in these domains.
KeywordsLipid Raft Adenylyl Cyclase Membrane Raft Pulmonary Artery Smooth Muscle Cell Caveolin Expression
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- Lee H, Woodman SE, Engelman JA et al (2001) Palmitoylation of caveolin-1 at a single site (Cys-156) controls its coupling to the c-Src tyrosine kinase: targeting of dually acylated molecules (GPI-linked, transmembrane, or cytoplasmic) to caveolae effectively uncouples c-Src and caveolin-1 (TYR-14). J Biol Chem 276:35150–35158PubMedCrossRefGoogle Scholar
- Murray F, Patel H, Suda R et al (2006) Caveolar localization and caveolin-1 regulation of PDE5 in human pulmonary artery smooth muscle cells. FASEB J. 20:A543Google Scholar
- Palade G (1953) Fine structure of blood capilaries. J Appl Physiol 24:1424–1436Google Scholar
- Volonte D, Galbiati F, Pestell RG et al (2001) Cellular stress induces the tyrosine phosphorylation of caveolin-1 (Tyr(14)) via activation of p38 mitogen-activated protein kinase and c-Src kinase. Evidence for caveolae, the actin cytoskeleton, and focal adhesions as mechanical sensors of osmotic stress. J Biol Chem 276:8094–8103PubMedCrossRefGoogle Scholar
- Younes A, Lyashkov AE, Graham D et al (2008) Ca 2+-stimulated basal adenylyl cyclase activity localization in membrane lipid microdomains of cardiac sinoatrial nodal pacemaker cells. J Biol Chem [ePub ahead of print 10.1074/jbc.M707540200]Google Scholar