Design and Function of a Light-Microscopy Facility

  • Kurt I. Anderson
  • Jeremy Sanderson
  • Jan Peychl
Part of the Principles and Practice book series (PRINCIPLES)

Modern biological research depends on a wide variety of specialized techniques, which collectively are beyond the grasp of a single research group. Research infrastructure, in the form of services and facilities, is therefore an increasingly important foundation for a competitive research institution. A lightmicroscopy facility is a place of dynamic interaction among users, staff, and equipment. Staff provide the organization, continuity, and expert knowledge required to manage the laser-safe interaction between demanding, selfish, high-performance users and delicate, expensive, high-performance equipment. They introduce novice users to fundamental principles of image acquisition and analysis, often beginning with fluorescence basics, but collaborate with advanced users in the development of new imaging techniques. Intimate knowledge of the experimental needs of the user research groups is required to maximize the effectiveness of equipment purchases, which are also informed by critical evaluation of local sales and support teams. Equipment management encompasses evaluation, purchase, installation, operation, and maintenance, and depends critically on good relations with competent local technical support. Special care should be given to the architectural design of an imaging facility to maximize the utility and comfort of the user environment and the long-term performance stability of the equipment. Finally, we present the details of a web-based equipment scheduling database as an essential organizational tool for running an imaging facility, and outline the important points underlying the estimation of hourly instrument costs in a fee-for-use setting.


Time Basis Support Ratio Cost Recovery Purchase Cost User Environment 
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  1. Alderson RH (1975) Design of the electron microscope laboratory. Practical methods in electron microscopy, vol 4. Elsevier, New York.Google Scholar
  2. Angeletti RN, Bonewald LF, de Jongh K, Niece R, Rush J, Stults J (1999) Research technologies: fulfilling the promise. FASEB J 13:595–601.PubMedGoogle Scholar
  3. DeMaggio S (2002) Running and setting up a confocal microscope core facility. Methods Cell Biol 70:475–485.CrossRefPubMedGoogle Scholar
  4. Helm JP, Haug F, Mogens S, Storm JF, Ottersen OP (2001) Design and installation of a multimode microscopy system. Proc SPIE 4262:396–406.
  5. Humphrey E (2004) How to promote a facility in order to increase use, acquire new equipment and, as a result, increase revenue. Microsc Today 12:32–36.Google Scholar
  6. Institut Pasteur (2007) PPMS: Pasteur platform management system for the PFID facility. Cited 2 May 2007.
  7. Murphy JA (1993) Designing a microscopy facility: step by step procedure. In: Robards AW, Wilson AJ (eds) Procedures in electron microscopy, vol 7. Wiley, Chichester, pp 1:1.1–1:1.23, 1:2.1–1:2.2.Google Scholar
  8. Murphy JA (2001) Image management for a multi-instrument, multi-platform microscopy facility. Scanning May.Google Scholar
  9. Murphy JA (2002) Designing a microscopy/analytical instrumentation facility: step by step procedure. Microsc Today 10:36–39.Google Scholar
  10. Sherman D (2003) Core facility management session: maintaining major equipment in the core microscopy facility. Microsc Today 11:40–45.Google Scholar
  11. University at Buffalo (1991)–archives. Cited 5 Dec 2006.
  12. White N, Errington R (2001) Setting up and managing a biological laser scanning microscope resource. Bio-Rad technical note 12 (reference no 9MRC50TN26).
  13. Winburn DC (1990). Practical laser safety. Dekker, New York.Google Scholar
  14. Zaluzec NT (1993) The Microscopy ListServer. Cited 5 Dec 2006.

Copyright information

© Springer-Verlag Berlin Heidelberg 2007

Authors and Affiliations

  • Kurt I. Anderson
    • 1
  • Jeremy Sanderson
    • 2
  • Jan Peychl
    • 2
  1. 1.Beatson Cancer Research InstituteGlasgowUK
  2. 2.Max Planck Institute for Molecular Cell Biology and GeneticsDresdenGermany

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