Viruses and Nanotechnology pp 1-21

Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 327)

Chemical Modification of Viruses and Virus-Like Particles

Protein capsids derived from viruses may be modified by methods, generated, isolated, and purified on large scales with relative ease. In recent years, methods for their chemical derivatization have been employed to broaden the properties and functions accessible to investigators desiring monodisperse, atomic-resolution structures on the nanometer scale. Here we review the reactions and methods used in these endeavors, including the modification of lysine, cysteine, and tyrosine side chains, as well as the installation of unnatural amino acids, with particular attention to the special challenges imposed by the polyvalency and size of virus-based scaffolds.

Abbreviations

CCMV:

Cowpea chlorotic mottle virus

CPMV:

Cowpea mosaic virus

DMSO:

Dimethyl sulfoxide

EDC:

1-Ethyl-3-(3-dimethyllaminopropyl)carb odiimide hydrochloride

HBA:

Hepatitis B virus

HSP:

Heat shock protein

MjHSP:

Methanococcus jannaschii heat shock protein

MMPP:

Magnesium monoper-oxyphthalate

MRI:

Magnetic resonance imaging

NHS:

N-hydroxysuccinimide

NɷV:

Nudaurelia capensis ɷ virus

RNA:

Ribonucleic acid

TMV:

Tobacco mosaic virus

TYMV:

Turnip yellow mosaic virus

UV:

Ultraviolet

VNP:

Viral nanoparticles

VLP

Virus-like particle

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© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  1. 1.Dynavax Technologies Corp.BerkeleyUSA
  2. 2.CB248, The Scripps Research InstituteLa JollaUSA

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