Nicotine Chemistry, Metabolism, Kinetics and Biomarkers

  • Neal L. Benowitz
  • Janne Hukkanen
  • Peyton JacobIII
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 192)


Nicotine underlies tobacco addiction, influences tobacco use patterns, and is used as a pharmacological aid to smoking cessation. The absorption, distribution and disposition characteristics of nicotine from tobacco and medicinal products are reviewed. Nicotine is metabolized primarily by the liver enzymes CYP2A6, UDPglucuronosyltransfease (UGT), and flavin-containing monooxygenase (FMO). In addition to genetic factors, nicotine metabolism is influenced by diet and meals, age, sex, use of estrogen-containing hormone preparations, pregnancy and kidney disease, other medications, and smoking itself. Substantial racial/ethnic differences are observed in nicotine metabolism, which are likely influenced by both genetic and environmental factors. The most widely used biomarker of nicotine intake is cotinine, which may be measured in blood, urine, saliva, hair, or nails. The current optimal plasma cotinine cut-point to distinguish smokers from non-smokers in the general US population is 3 ng ml−1. This cut-point is much lower than that established 20 years ago, reflecting less secondhand smoke exposure due to clear air policies and more light or occasional smoking.


Nicotine Replacement Therapy Grapefruit Juice Environmental Tobacco Smoke Exposure Nicotine Concentration Nicotine Intake 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Neal L. Benowitz
    • 1
  • Janne Hukkanen
    • 2
    • 3
  • Peyton JacobIII
    • 2
    • 3
  1. 1.Division of Clinical Pharmacology and Experimental TherapeuticsUniversity of CaliforniaSan FranciscoUSA
  2. 2.Division of Clinical Pharmacology and Experimental Therapeutics, Medical ServiceSan Francisco General Hospital Medical CenterSan FranciscoUSA
  3. 3.The Departments of Medicine and Biopharmaceutical SciencesUniversity of CaliforniaSan FranciscoUSA

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