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Soft X-Ray Absorption Imaging of Whole Wet Tissue Culture Cells

  • Conference paper
X-Ray Microscopy III

Part of the book series: Springer Series in Optical Sciences ((SSOS,volume 67))

Abstract

Using the Stony Brook/NSLS Scanning Transmission X-Ray Microscope (STXM), we have produced high resolution images of whole, wet, fixed and unfixed fibroblasts. Images of unfixed cells were taken at a wavelength of λ=34.1Å. An image can take 5 minutes to achieve a 14:1 background noise. The x-ray dosage, required to make such an image on wet initially live fibroblasts does not cause immediate observable structural damage to the target cells.

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References

  1. H. Rarback, C.J. Buckley, K. Goncz, H. Ade, E. Anderson, D. Attwood, P. Batson, S. Hellman, C. Jacobsen, D. Kern, J. Kirz, S. Lindaas, I. Mcnulty, M. Oversluizen, M. Rivers, S. Rothman, D. Shu, and E. Tang: NIM A291, 54–59 (1990)

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Author information

Authors and Affiliations

  1. Division of Physics, Mathematics and Astronomy, California Institute of Technology, Pasadena, CA, 91125, USA

    J. R. Gilbert & J. Pine

  2. Physics Department, State University of New York, Stony Brook, NY, 11794, USA

    J. Kirz, C. Jacobsen & S. Williams

  3. Physics Department, King’s College London, Strand, London, WC2R 2LS, UK

    C. J. Buckley

  4. National Synchrotron Light Source, Brookhaven National Laboratory, Upton, NY, 11973, USA

    H. Rarback

Authors
  1. J. R. Gilbert
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  2. J. Pine
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  3. J. Kirz
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  4. C. Jacobsen
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  5. S. Williams
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  6. C. J. Buckley
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  7. H. Rarback
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Editor information

Editors and Affiliations

  1. Physics Department, King’s College London, The Strand, WC2R 2LS, London, UK

    Alan G. Michette Ph. D. , Graeme R. Morrison Ph. D.  & Chistopher J. Buckley Ph. D. ,  & 

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© 1992 Springer-Verlag Berlin Heidelberg

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Gilbert, J.R. et al. (1992). Soft X-Ray Absorption Imaging of Whole Wet Tissue Culture Cells. In: Michette, A.G., Morrison, G.R., Buckley, C.J. (eds) X-Ray Microscopy III. Springer Series in Optical Sciences, vol 67. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-46887-5_87

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  • DOI: https://doi.org/10.1007/978-3-540-46887-5_87

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-13894-6

  • Online ISBN: 978-3-540-46887-5

  • eBook Packages: Springer Book Archive

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