Alcian Blue Staining Technique for Tracing the Governing Vessel (GV) Meridian in Rat

  • Min-Su Kim
  • Vyacheslav Ogay
  • Yong-Hui Han
  • Hak-Soo Shin
  • Byung-Cheon Lee
  • Kwang-Sup Soh
Conference paper

DOI: 10.1007/978-3-540-36841-0_904

Part of the IFMBE Proceedings book series (IFMBE, volume 14)
Cite this paper as:
Kim MS., Ogay V., Han YH., Shin HS., Lee BC., Soh KS. (2007) Alcian Blue Staining Technique for Tracing the Governing Vessel (GV) Meridian in Rat. In: Magjarevic R., Nagel J.H. (eds) World Congress on Medical Physics and Biomedical Engineering 2006. IFMBE Proceedings, vol 14. Springer, Berlin, Heidelberg

Abstract

There has been much effort in search for the anatomical structure corresponding to acupuncture meridians without success. For this work one needs specific dyes that stain exclusively the tissues of acupuncture meridians. Until the present time no one was able to find the necessary dyes except Bong Han Kim who claimed finding such a dye to trace all the meridians. Unfortunately he kept this dye secret and no one was able to reproduce his result. Only very recently we found a staining dye, Alcian blue that stains the acupuncture meridian and were able to trace the governing vessel (GV) of a rat using this dye. After injection of Alcian blue at LSP (lumbar sacral point) of the GV meridian, we observed a blue line at the GV meridian above loose connective tissue. The dye flowed along the meridian from the acupuncture point, while it spread by diffusion process when injected at non-acupoints. The dye stains hyaluronic acid which is known to be highly concentrated in Bonghan duct system. Thus our observation is in agreement with Bonghan theory, and can be a starting point of tracing all other meridians.

Keywords

Acupuncture meridian Alcian blue Bonghan duct system Lumbar sacral point 

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Copyright information

© International Federation for Medical and Biological Engineering 2007

Authors and Affiliations

  • Min-Su Kim
    • 1
  • Vyacheslav Ogay
    • 1
  • Yong-Hui Han
    • 1
  • Hak-Soo Shin
    • 1
  • Byung-Cheon Lee
    • 1
  • Kwang-Sup Soh
    • 1
  1. 1.Biomedical Physics Laboratory, Department of PhysicsSeoul National UniversitySeoulKorea
  2. 2.Seoul National UniversitySeoulKorea

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