Online Sauter Diameter Measurement of Air Bubbles and Oil Drops in Stirred Bioreactors by Using Hough Transform
Industrial production of important fermentation products such as enzymes, antibiotics, and aroma compounds, amongst others, involves a multiphase dispersion. Therefore, it is important to determine the influence of the bioreactor operational parameters (stirring speed, impeller type, power drawn, etc.) under which the culture achieves the highest yields. The automatic on-line analysis of multiphase dispersions occurring in microbial cultures in mechanically stirred bioreactors, presents a number of important problems for image acquisition and segmentation, including heterogeneous transparency of moving objects of interest and background, blurring, overlapping and artifacts. In this work, a Hough transform based method was implemented and tested. Results were compared with those obtained manually by the expert. We concluded that using this method, the evaluation of size distributions of air bubbles and oil drops in a mechanically stirred bioreactor was performed in a more efficient and less time-consuming way than others semiautomatic or manual methods.
Unable to display preview. Download preview PDF.
- 8.Zhou, Z., Xu, Z., Masliyah, J., Kasongo, T., Christendat, D., Hyland, K., Kizor, T., Cox, D.: Application of on-line visualization to flotation system. In: Proc. Of the 32nd Annual Operator’s Conferences of the Canadian Mineral processors, pp. 120–137 (2000)Google Scholar
- 11.So, S., Morikita, H., Takagi, S., Matsumoto, Y.: Laser doppler velocimetry measurement of turbulent bubbly chanel flow. Experimentes in Fluids 33, 135–142 (2002)Google Scholar
- 13.Taboada, B., Larralde, P., Brito, T., Vega -Alvarado, L., Díaz, R., Galindo, E., Corkidi, G.: Images Acquisition of a Multiphase Dispersions in Fermentation Processes. Journal of Applied Research and Technology 1(1), 78–84 (2003)Google Scholar
- 14.Image-Pro Plus V.4.1, Reference Guide for Windows (Media Cybernetics,EUA) (1999)Google Scholar
- 15.Hough, P.V.C.: Methods and means for recognizing complex patterns, U.S Patent 3, 069, 654 (1962)Google Scholar
- 16.Kass, W.A., Terzopoulos, D.: Snakes: Active contour models. In: Porceed. Of first International Conf. On Comp. Vis. pp. 259–269 (1987)Google Scholar
- 21.Pacek, C., Man, C., Nienow, A.: Chemical Engineering. Science 53(11), 2005–2011 (1998)Google Scholar