Applications of Image Registration in Human Genome Research
Fluorescence microscopy has some limitations and imperfections that can affect some types of study in human genome research. Image registration methods can help overcome or reduce the effect of some of them. This paper is concentrated on several applications of image registration that are carried out in our laboratory. The concerned areas are: 1. chromatic aberration correction, which improves precision of colocalization studies, 2. registration of images after repeated acquisitions, which helps to enlarge the number of object types studied simultaneously, and 3. registration of tilted images in micro-axial tomography, which helps to improve optical resolution of light microscopy.
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- 1.Cremer, T., Kurz, A., Zirbel, R., Dietzel, S., Rinke, B., et al.: Role of chromosome territories in the functional compartmentalization of the cell nucleus. Cold Spring Harbor Symp. Quantitative Biology 58, 777–792 (1993)Google Scholar
- 2.Eils, R., Bertin, E., Saracoglu, K., Rinke, B., Schröck, E., Parazza, F., Usson, Y., Robert-Nicoud, M., Stelzer, E.H.K., Chassery, J.M., Cremer, T., Cremer, C.: Application of confocal laser microscopy and 3D Voronoi diagrams for volume and surface estimates of interphase chromosomes. J. Microsc. 177, 150–161 (1995)Google Scholar
- 3.Lukášová, E., Kozubek, S., Kozubek, M., Kroha, V., Marečková, A., Skalníková, M., Bártová, E., Slotová, J.: Chromosomes participating in translocations typical of malignant haemoblastosis are also involved in exchange aberrations induced by fast neutrons. Radiation Research 151, 375–384 (1999)CrossRefGoogle Scholar
- 9.Matula, P., Kozubek, M., Steier, F., Hausmann, M.: Precise 3D image alignment in micro axial tomography. J. Microsc. 209 (2003)Google Scholar
- 11.Cogswell, C.J., Larkin, K.G., Klemm, H.: Fluorescence microtomography: Multiangle image acquisition and 3D digital reconstruction. In: SPIE, vol. 2655, pp. 109–115 (1996)Google Scholar