Using Stem Cell-Derived Microvesicles in Regenerative Medicine: A New Paradigm for Cell-Based-Cell-Free Therapy

  • Mohammad Amin Rezvanfar
  • Mohammad Abdollahi
  • Fakher Rahim
Part of the Stem Cells in Clinical Applications book series (SCCA)


The current advances in the field of stem cell-based therapies have paved the way for these novel treatments to enter clinical trials. Among them, mesenchymal stem cell (MSC) based therapies have offered an important potential modality of regenerative medicine and immunotherapy. MSCs are multipotent/pluripotent fibroblast-like cells that exist in almost all tissues and have the potential to differentiate into various cell types from all three germ layers (i.e., ectoderm, mesoderm, and endoderm). The regenerative ability of MSCs might be attributed to their paracrine actions on neighboring host cells rather than a trans-differentiation into the tissue specific cells. These paracrine factors are contained in endosomal derived extracellular microvesicles (EVs), which are released at the site of injury, where they secrete large quantities of bioactive factors like proteins, mRNAs, and miRNAs with anti-inflammatory, antioxidant, trophic, antiapoptotic, and angiogenic effects. Recent findings, however, have demonstrated remarkable therapeutic effects and regenerative potential of MSC-derived EVs showing that conditioned media from stem cell cultures can produce similar efficacious effects compared to those observed for cells. In line with these findings, in a series of experiments our research teams have demonstrated that MSC-derived EVs served as trophic shuttles for enhancing sperm quality parameters and reducing complications of multiple sclerosis. As a bilipid membrane vesicle with many membrane-bound proteins and a diverse cargo, exosomes represents an ideal therapeutic agent that have the potential to home and target tissues and treat complicated diseases such as cardiovascular injuries, chemotherapy side effects, infertility, and MS. In this chapter, the latest evidence on the beneficial effects of the stem cell-derived EVs is explored to support the development of clinical grade bioproducts as new GMP-based cell-free regenerative medicines in tissue regeneration.


Stem cells Extracellular microvesicles (Evs) Exosomes Disease Cell-free biomedicine 


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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Mohammad Amin Rezvanfar
    • 1
  • Mohammad Abdollahi
    • 1
    • 2
  • Fakher Rahim
    • 3
  1. 1.Department of Toxicology and Diseases, Pharmaceutical Sciences Research Center (PSRC)Tehran University of Medical Sciences (TUMS)TehranIran
  2. 2.Department of Toxicology and Pharmacology, Faculty of PharmacyTehran University of Medical Sciences (TUMS)TehranIran
  3. 3.Health Research Institute, Research Center of Thalassemia and HemoglobinopathiesAhvaz Jundishapur University of Medical SciencesAhvazIran

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