Advertisement

Applications of AIEgens in Super-Resolution Imaging, Fluorescence Lifetime Imaging, and Fluorescence Anisotropy Imaging

  • Engui Zhao
  • Sijie Chen
Chapter

Abstract

Aggregation-induced emission (AIE) luminogens (AIEgens) are emerging as a new class of luminescent materials for bioimaging. They are usually with excellent optical properties, appreciable biocompatibility and thus have been used extensively for cell imaging and sensing by employing conventional fluorescence microscopy and confocal microscopy. Recently, their applications in advanced imaging techniques such as super-resolution imaging, fluorescence lifetime imaging (FLIM), and fluorescence anisotropy imaging (FAIM) have been explored and successfully demonstrated. In this chapter, we select some representative examples to illustrate the explorations and discoveries in this area.

Keywords

Aggregation-induced emission (AIE) Super-resolution imaging Stochastic optical reconstruction microscopy (STORM) Stimulated emission depletion (STED) microscopy Fluorescence lifetime imaging (FLIM) Fluorescence anisotropy imaging (FAIM) 

References

  1. 1.
    Yu J, Sun X, Cai F et al (2015) Low photobleaching and high emission depletion efficiency: the potential of AIE luminogen as fluorescent probe for STED microscopy. Opt Lett 40:2313.  https://doi.org/10.1364/OL.40.002313CrossRefGoogle Scholar
  2. 2.
    Li D, Qin W, Xu B et al (2017) AIE nanoparticles with high stimulated emission depletion efficiency and photobleaching resistance for long-term super-resolution bioimaging. Adv Mater 29:1–9.  https://doi.org/10.1002/adma.201703643CrossRefGoogle Scholar
  3. 3.
    Fang X, Chen X, Li R et al (2017) Multicolor photo-crosslinkable aiegens toward compact nanodots for subcellular imaging and sted nanoscopy. Small 13:1–7.  https://doi.org/10.1002/smll.201702128CrossRefGoogle Scholar
  4. 4.
    Rust MJ, Bates M, Zhuang X (2006) imaging by stochastic optical reconstruction microscopy (STORM). Nat Methods 3:793–796.  https://doi.org/10.1038/NMETH929CrossRefGoogle Scholar
  5. 5.
    Fernández-Suárez M, Ting AY (2008) Fluorescent probes for super-resolution imaging in living cells. Nat Rev Mol Cell Biol 9:929–943.  https://doi.org/10.1038/nrm2531CrossRefGoogle Scholar
  6. 6.
    Li C, Gong W-L, Hu Z et al (2013) Photoswitchable aggregation-induced emission of a dithienylethene–tetraphenylethene conjugate for optical memory and super-resolution imaging. RSC Adv 3:8967.  https://doi.org/10.1039/c3ra40674aCrossRefGoogle Scholar
  7. 7.
    Gu X, Zhao E, Zhao T et al (2016) A mitochondrion-specific photoactivatable fluorescence turn-on aie-based bioprobe for localization super-resolution microscope. Adv Mater 28(25):5064–5071.  https://doi.org/10.1002/adma.201505906CrossRefGoogle Scholar
  8. 8.
    Lo CYW, Chen S, Creed SJ et al (2016) Novel super-resolution capable mitochondrial probe, MitoRed AIE, enables assessment of real-time molecular mitochondrial dynamics. Sci Rep 6:1–9.  https://doi.org/10.1038/srep30855CrossRefGoogle Scholar
  9. 9.
    Zhao N, Chen S, Hong Y, Tang BZ (2015) A red emitting mitochondria-targeted AIE probe as an indicator for membrane potential and mouse sperm activity. Chem Commun 51:13599–13602.  https://doi.org/10.1039/C5CC04731ECrossRefGoogle Scholar
  10. 10.
    Hong Y, Lam JWY, Tang BZ (2009) Aggregation-induced emission: phenomenon, mechanism and applications. Chem Commun (29):4332–4353.  https://doi.org/10.1039/b904665h
  11. 11.
    Gao M, Hong Y, Chen B et al (2017) AIE conjugated polyelectrolytes based on tetraphenylethene for efficient fluorescence imaging and lifetime imaging of living cells. Polym Chem 8:3862–3866.  https://doi.org/10.1039/C7PY00564DCrossRefGoogle Scholar
  12. 12.
    Chen S, Hong Y, Zeng Y et al (2015) Mapping live cell viscosity with an aggregation-induced emission fluorogen by means of two-photon fluorescence lifetime imaging. Chem Eur J 21:4315–4320.  https://doi.org/10.1002/chem.201405658CrossRefGoogle Scholar
  13. 13.
    Soleimaninejad H, Chen MZ, Lou X et al (2017) Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen. Chem Commun 53:2874–2877.  https://doi.org/10.1039/C6CC09916ECrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Hong KongChina
  2. 2.Ming Wai Lau Centre for Reparative Medicine, Karolinska InstitutetHong KongChina

Personalised recommendations