Fluorescent Aptasensor Based on Aggregation-Induced Emission Probe and Carbon nanomaterials

  • Lian Ma
  • Ke Ma
  • Bin Xu
  • Wenjing TianEmail author


Fluorescent biosensors have received great attention because of their high sensitivity, rapid and easy operations. Recently, fluorescent probes with aggregation-induced emission (AIE) feature provide a new approach for label-free and turn-on fluorescent analysis assay, due to their unique luminescent characteristics. Here, we summarized the recent progress of fluorescent aptasensor for biomolecules based on the integration of AIE probe and carbon nanomaterials. The introduction of carbon nanomaterials can not only enhance the sensitivity, but also improve the selectivity for biomolecules. Through optimizing the supramolecular interactions of AIE probes and carbon nanomaterials with biomolecules, the detection limitation can reach as low as 0.17 nM for detecting the target DNA sequence. It is believed that the research efforts will provide an efficient approach to improve the performance of biomolecules sensing assay and an in-depth understanding of the supramolecular interactions of AIE probes and carbon nanomaterials with biomolecules, and thus facilitate their extended applications in biosensors and biomedicine.


Fluorescence Aptasensor AIE Carbon nanomaterials Supramolecular interactions 


  1. 1.
    Demchenko AP (2009) Introduction to fluorescence sensing. Springer, New YorkCrossRefGoogle Scholar
  2. 2.
    Sauer M (2003) Single-molecule-sensitive fluorescent sensors based on photoinduced intramolecular charge transfer, vol 42. Wiley, Chichester, pp 1790–1793Google Scholar
  3. 3.
    Wang M et al (2010) Fluorescent bio/chemosensors based on silole and tetraphenylethene luminogens with aggregation-induced emission feature. RSC Adv 20:1858Google Scholar
  4. 4.
    Luo J et al (2001) Aggregation-induced emission of 1-methyl-1, 2, 3, 4, 5-pentaphenylsilole. RSC Adv 18:1740–1741Google Scholar
  5. 5.
    Li X et al (2013) Label-free fluorescence turn-on detection of Pb2+ based on AIE-active quaternary ammonium salt of 9, 10-distyrylanthracene. RSC Adv 5(2):438–441Google Scholar
  6. 6.
    Li X et al (2014) Highly sensitive determination of ssDNA and real-time sensing of nuclease activity and inhibition based on the controlled self-assembly of a 9, 10-distyrylanthracene probe. Anal Bioanal Chem 406(3):851–858CrossRefGoogle Scholar
  7. 7.
    Ma K et al (2014) A sensitive and selective “turn-on” fluorescent probe for Hg 2+ based on thymine–Hg 2+–thymine complex with an aggregation-induced emission feature. RSC Adv 6(7):2338–2342Google Scholar
  8. 8.
    Wang ZL et al (2013) A highly sensitive “turn-on” fluorescent probe for bovine serum albumin protein detection and quantification based on AIE-active distyrylanthracene derivative. Sci China Chem 56(9):1234–1238CrossRefGoogle Scholar
  9. 9.
    Santhosh C et al (2016) Role of nanomaterials in water treatment applications: a review. Chem Eng J 306:1116–1137CrossRefGoogle Scholar
  10. 10.
    Li Q, Li Z (2015) AIE probes towards biomolecules: the improved selectivity with the aid of graphene oxide. Sci China Chem 58(12):1800–1809CrossRefGoogle Scholar
  11. 11.
    Xu X et al (2011) A graphene oxide-based AIE biosensor with high selectivity toward bovine serum albumin. RSC Adv 47(45):12385–12387Google Scholar
  12. 12.
    Balapanuru J et al (2010) A graphene oxide–organic dye ionic complex with DNA-sensing and optical-limiting properties. Angew Chem Int Ed Engl 122(37):6699–6703CrossRefGoogle Scholar
  13. 13.
    Li X et al (2013) Fluorescent aptasensor based on aggregation-induced emission probe and graphene oxide. ACS Adv 86(1):298–303Google Scholar
  14. 14.
    Wang H et al (2016) Tunable supramolecular interactions of aggregation-induced emission probe and graphene oxide with biomolecules: an approach toward ultrasensitive label-free and “turn-on” DNA sensing. Small 12(47):6613–6622CrossRefGoogle Scholar
  15. 15.
    Xu X et al (2012) A strategy for dramatically enhancing the selectivity of molecules showing aggregation-induced emission towards biomacromolecules with the aid of graphene oxide. Chemistry 18(23):7278–7286CrossRefGoogle Scholar
  16. 16.
    Zhang R et al (2017) Real-time naked-eye multiplex detection of toxins and bacteria using AIEgens with the assistance of graphene oxide. RSC Adv 196:363–375Google Scholar
  17. 17.
    Kwok RTK et al (2014) Water-soluble bioprobes with aggregation-induced emission characteristics for light-up sensing of heparin. RSC Adv 2(26):4134–4141Google Scholar
  18. 18.
    Ou X et al (2017) A highly sensitive and facile graphene oxide-based nucleic acid probe: label-free detection of telomerase activity in cancer patient’s urine using AIEgens. Biosens Bioelectron 89:417–421CrossRefGoogle Scholar
  19. 19.
    Zhu Z et al (2010) Single-walled carbon nanotube as an effective quencher. Anal Bioanl Chem 396(1):73–83CrossRefGoogle Scholar
  20. 20.
    Ma K et al (2017) Label-free detection for SNP using AIE probes and carbon nanotubes. Sens Actuator B Chem 253:92–96CrossRefGoogle Scholar
  21. 21.
    Ma K et al (2016) A label-free aptasensor for turn-on fluorescent detection of ATP based on AIE-active probe and water-soluble carbon nanotubes. Sens Actuator B Chem 230:556–558CrossRefGoogle Scholar

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Supramolecular Structure and MaterialsJilin UniversityChangchunChina

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