Specimen Handling and Optimal Processing

  • César R. Lacruz
  • Javier Saénz de Santamaría
  • Ricardo H. Bardales
Part of the Essentials in Cytopathology book series (EICP, volume 13)


Retrospective review of diagnostic errors in CNS intraoperative consultation often reveals that diagnosis was based on inadequate tissue sampling or subpar preparations. Therefore, the correct performance of the whole process should include proper identification of the specimen, appropriate transportation, adequate tissue sampling, careful achievement of the squash/smearing technique, and quick, but optimal, fixation and staining. In this chapter, these important topics are discussed including detailed information about the fast staining methods more often used.


Intraoperative brain assessment Cytology Squash preparation Smear preparation Touch preparation Fast staining methods Specimen handling Specimen processing 

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • César R. Lacruz
    • 1
  • Javier Saénz de Santamaría
    • 2
  • Ricardo H. Bardales
    • 3
  1. 1.Professor of PathologyComplutense University School of MedicineMadridSpain
  2. 2.Professor of PathologyUniversity Hospital Extremadura, Medical SchoolBadajozSpain
  3. 3.Pathologist, Director Ultrasound-guided Fine Needle Aspiration ServiceOutpatient Pathology Associates / Precision PathologySacramentoUSA

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