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In Vitro Digestibility of Native Grass Silage Supplemented with Leucaena leucocephala

  • Ryan Aryadin Putra
  • Cuk Tri Noviandi
  • Nafiatul Umami
Conference paper

Abstract

Research has been conducted to determine the best level of Leucaena leucocephala supplementation to digestibility and fermentability in vitro. The study was designed using completely randomized design with three levels supplementation of Leucaena (10%, 20%, and 30%) and each treatment was done in six replications. All of the silage treatments was inoculated with 2% Lactobacillus plantarum, and stored in mini-silos for 21 days. The results showed that supplementing native grass with 20% Leucaena resulted in the most significant (P < 0.05) dry matter and organic matter digestibility. The highest fiber crude digestibility was obtained at 30% supplementation. However, the highest digestibility of crude protein obtained at 10% Leucaena supplementation and the digestibility decreased linearly with the increasing levels of supplementation (P < 0.05). These data indicate that supplementing native grass with 20% Leucaena is the best treatment in terms of nutrient digestibility.

Keywords

Leucaena leucocephala Lactobacillus plantarum In vitro Native grass Silage 

Abbreviations

CF

crude fiber

CFD

crude fiber digestibility

CP

crude protein

CPD

crude protein digestibility

DM

dry matter

DMD

dry matter digestibility

EE

Ether Extract

IVCFD

in vitro crude fiber digestibility

IVCPD

in vitro crude protein digestibility

IVDMD

in vitro dry matter digestibility

IVOMD

in vitro organic matter digestibility

LAB

lactic acid bacteria

LIPI

Lembaga Ilmu Pengetahuan Indonesia

NFE

Nitrogen free extract

OM

organic matter

OMD

organic matter digestibility

PEG

polyethylene glycol

TDN

total digestible nutrient

UGM

Universitas Gadjah Mada

Notes

Acknowledgments

Thanks to Indonesia Endowment Fun for Education, the Ministry of Finance, The Republic of Indonesia as the main sponsor of this research, either in the financial support and the moral support. (Kep-105/LPDP/2014)

References

  1. 1.
    Yasin S. Perilaku makan ruminansia sebagai bioindikator fenologi dan dinamika padang penggembalaan [Ingestive behaviour of ruminant as bioindicator of phenology and dynamics of grassland]. Pastura 2013;3(1):1–4. (in Bahasa Indonesia).Google Scholar
  2. 2.
    National Research Council. Nutrient requirements of beef cattle. 7th rev. ed. Washington, DC: The National Academies Press; 1996.
  3. 3.
    Souza WF, Pereira OG, Ribeiro KG, Santos SA, Valadares-Filho SC. Intake, digestibility, nitrogen efficiency, and animal performance of growing and finishing beef cattle fed warm-season legume (Stylosanthes capitata plus Stylosanthes macrocephala) silage replacing corn silage. J Anim Sci. 2014;92(9):4099–107.CrossRefPubMedGoogle Scholar
  4. 4.
    Gusha J, Halimani TE, Ngongoni NT, Ncube S. Effect of feeding cactus-legum silages on nitrogen retention, digestibility and microbial protein synthesis in goats. Anim Feed Sci Technol. 2015;206:1–7.CrossRefGoogle Scholar
  5. 5.
    Yunus M. Pengaruh pemberian daun lamtoro (Leucaena leucocephala) terhadap kualitas silase rumput gajah (Pennisetum purpereum) yang diberi molasses [The effect of gift lamtoro leaf (Leucaena leocephala) to quality of silage of elephant grass (Pennisetum purpereum) that adding molasses)]. Agripet. 2009;9:38–42. (in Bahasa Indonesia).Google Scholar
  6. 6.
    Association of Official Analytical Chemist. Official method of analysis. 18th ed. Washington, DC: The Association of Official Analytical Chemist; 2005.Google Scholar
  7. 7.
    Tilley JMA, Terry RA. A two-stage technique for the in vitro digestion of forage crop. J Br Grassl Soc. 1963;18:104–11.CrossRefGoogle Scholar
  8. 8.
    Makkar HPS. In vitro screening of feed resources for efficiency of microbial protein synhesis (Chapter 7). In: Vercoe PE, Makkar HPS, Schlink AC, editors. In vitro screening of plant resources for extra-nutritional attributes in ruminants: nuclear and related methodologies. Netherlands: Springer; 2010. p. 107–144.Google Scholar
  9. 9.
    Hartadi H, Reksohadiprodjo S, Tillman AD. Tabel komposisi pakan untuk Indonesia [Indonesian feed composition tables]. Yogyakarta: UGM Press; 2005. (in Bahasa Indonesia).Google Scholar
  10. 10.
    Tan HY, Sieo CC, Abdullah N, Liang JB, Huang XD, Ho YW. Effects of condensed tannins from Leucaena on methane production, rumen fermentation, and populations of methanogens and protozoa in vitro. Anim Feed Sci Technol. 2011;169:185–93.CrossRefGoogle Scholar
  11. 11.
    Niderkorn V, Mueller-Harvey I, Morvan AL, Aufrere J. Synergistic effects of mixing cocksfoot and sainfoin on in vitro rumen fermentation. Role of condensed tannins. Anim Feed Sci. 2012;178(1–2):48–56.CrossRefGoogle Scholar
  12. 12.
    Galindo J, Marrero Y, Ruiz TE, González N, Díaz A, Aldama AI, et al. Effect of a multiple mixture of herbaceous legumes and Leucaena leucocephala on the microbial population and fermentative products in the rumen of Zebu upgraded yearling steers. Cuba J Agric Sci. 2009;43:251–7.Google Scholar
  13. 13.
    Min BR, Attwood GT, Reilly K, Sun W, Peters JS, Barry TN, et al. Lotus corniculatus condensed tannins decrease in vivo populations of proteolytic bacteria and affect nitrogen metabolism in the rumen of sheep. Can J Anim Sci. 2002;48:911–21.Google Scholar
  14. 14.
    Toral PG, Hervas G, Missaoui H, Andres S, Giraldez FJ, Jellali S, et al. Effects of a tannin-rich legume (Onobrychis viciifolia) on in vitro ruminal biohydrogenation and fermentation. Span J Agric Res. 2016;14(1):e0602.  https://doi.org/10.5424/sjar/2016141-8989.CrossRefGoogle Scholar
  15. 15.
    McDonald P, Edwards RA, Greenhalgh JFD, Morgan CA, Sinclair LA, Wilkinson RG. Animal nutrition. 7th ed. London: Prentice Hall; 2011.Google Scholar
  16. 16.
    Goel G, Puniya AK, Aguilar CN, Singh K. Review: interaction of gut microflora with tannins in feed. Naturwissenchaften. 2005;92:497–503.CrossRefGoogle Scholar
  17. 17.
    Barros-Rodríguez MA, Solorio-Sánchez FJ, Sandoval-Castro CA, Klieve A, Rojas-Herrera RA, Briceño-Poot EG, et al. Rumen function in vivo and in vitro in sheep fed Leucaena leucocephala. Trop Anim Health Prod. 2015;47:757–64.CrossRefPubMedGoogle Scholar
  18. 18.
    Tillman AD, Hartadi H, Reksohadiprodjo S, Lebdosoekojo S. Ilmu makanan ternak dasar [Principle of animal nutrition]. Yogyakarta: UGM Press; 1991. (in Bahasa Indonesia).Google Scholar

Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Ryan Aryadin Putra
    • 1
  • Cuk Tri Noviandi
    • 1
  • Nafiatul Umami
    • 1
  1. 1.Faculty of Animal ScienceUniversitas Gadjah MadaYogyakartaIndonesia

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