Advertisement

Biosorption of Metals Ion on Methanol Dehydrogenase Activity Test of Bradyrhizobium japonicum USDA110

  • Novita Kurniawati
  • Ambar Pertiwiningrum
  • Yuny Erwanto
  • Nanung Agus Fitriyanto
  • Mohammad Zainal Abidin
Conference paper

Abstract

This research aimed to know effect of absorption metals ion (bisorption) which has different group elements on methanol dehydrogenase (MDH) activity test of a nitrogen fixation bacterium, Bradyhizobium japonicum USDA 110. Ten of metals ion at a concentration of 30 μM were tested to grow B. japonicum USDA 110 in diluted one per ten of nutrient medium. The results of MDH activity test showed that the bacterial growth in medium without added metal ion had similar MDH activity with that of in the medium supplemented with calsium ion/Ca2+. Highest enzymatic activity, 0.08 (U · mg−1), was showed by the bacterium grown in medium supplemented with magnesium/Mg2 + .. MDH was required for the bacterial growth in anerobic condition specially for a root simbiotic bacterium, Bradyhizobium japonicum USDA 110. Addition of magnesium/Mg2+ accelerated the bacterial growth and MDH activity by 2.6 and 1.28 times, respectively. This research results deduced that in an anerobic condition of soil, the metal ions specially calsium and magnesium were required to be added in plant growth medium to accelerate the growth of B. japonicum which well known as bacterial symbiotic on soy bean roots.

Keywords

Biosorption Bradyrhizobium japonicum USDA110 Metal ion Methanol dehydrogenase Nutrient medium 

Abbreviations

BSA

bovine serum albumin

DCPIP

Dichlorophenolindophenol

MDH

Methanol Dehydrogenase

NBT

Nitro Blue Tetrazolium

PAGE

Polyacrylamide Gel Electrophoresis

PES

Phenazine Ethosulfate

SDS

Sodium Dodecyl sulfat

YM

Yeast extrac-Mannitol

Notes

Acknowledgments

The author gratitude to the directorate General of Higher Education, Ministry of Research, Technology, and Higher Education of the Republic of Indonesia which endoswed her Master study program scholarship.

References

  1. 1.
    Marshall MN, MacDonald RC, Franzen JJ, Wojciechowski C, Fall R. Methanol emission from leaves: enzymatic detection of gas-phase methanol and relation of methanol fluxes to stomatal conductance and leaf development. Plant Physiol. 1995;108:1359–68.CrossRefGoogle Scholar
  2. 2.
    Sadowsky MJ, Bohlool BB. Growth of fast- and slow-growing rhizobia on ethanol. Appl Environ Microbiol. 1986;52:951–3.PubMedPubMedCentralGoogle Scholar
  3. 3.
    Kykendall LD, Elkan GH. Rhizobium japonicum derivatives differing in nitrogen-fixing efficiency and carbohydrate utilization. Appl Environ Microbiol. 1976;32:511–9.Google Scholar
  4. 4.
    Kaczynka G, Borowik A, Wyszkowska J. Soil dehydrogenase as an indicator of the environment with petroleum products. Water Air Soil Polllut. 2015;226:372.CrossRefGoogle Scholar
  5. 5.
    Skovran E, Palmer AD, Rountree AM, Good NM, Lidstrom ME. XoxF is required for expression of methanol dehydrogenase in Methylobacterium extorquens AM1. J Bacteriol. 2011;193:6032–8.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Saito A, Mitsui H, Hattori R, Minamisawa K, Hattori T. Slow-growing and oligotrophic soil bacteria phylogenetically close to Bradyrhizobium japonicum. FEMS Microbiol Ecol. 1998;25:277–86.CrossRefGoogle Scholar
  7. 7.
    Day DJ, Anthony C. Methanol dehydrogenase from Methylobacterium extorquens AM1. Methods Enzymol. 1990;188:210–6.CrossRefGoogle Scholar
  8. 8.
    Goodwin MG, Anthony C. Characterization of a novel methanol dehydrogenase containing a Ba2+ ion at the active site. Biochem J. 1996;318:673–9.CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Chistoserdova L, Lidstrom ME. Molecular and mutational analysis of a DNA region separating two methylotrophy gene clusters in Methylobacterium extorquens AM1. Microbiology. 1997;143:1729–36.CrossRefPubMedGoogle Scholar
  10. 10.
    Casida LE. Microbial metabolic activity in soil as measured by dehydrogenase determinations. Appl Environ Microbiol. 1977;34:630–6.PubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Novita Kurniawati
    • 1
  • Ambar Pertiwiningrum
    • 1
  • Yuny Erwanto
    • 1
  • Nanung Agus Fitriyanto
    • 1
  • Mohammad Zainal Abidin
    • 1
  1. 1.Departement of Animal Products Technology, Faculty of Animal ScienceUniversitas Gadjah MadaYogyakartaIndonesia

Personalised recommendations