Sample Acquisition and Test Requisition

  • Ross A. Miller
  • Ashwyna Sunassee


In the modern era of cytopathology, sample acquisition refers to the process of procuring specimen material for both cytopathologic examination and ancillary testing. Depending on the site, sample acquisition can be categorized as either exfoliative or aspiration which refers to how material is obtained. Fine-needle aspiration (FNA) is a type of aspiration specimen procurement used to target a specific area not amenable to exfoliative techniques. Mastery of the FNA technique is critical in order to obtain optimal material for cytopathologic examination and further testing. Often, rapid on-site evaluation (ROSE) is utilized in aspiration cytopathology which allows for on-site and real-time specimen adequacy evaluation and allows for appropriate specimen triage during the procedure. After specimen collection, an appropriate test requisition should accompany the specimen to the cytopathology laboratory. This ensures the correct sample is matched with the correct patient and that the sample is appropriately handled.


Sample acquisition Fine-needle aspiration Rapid on-site evaluation Test requisition Ancillary testing 



Core-needle biopsy


Endobronchial ultrasound


Endoscopic ultrasound


Fine-needle aspiration


Rapid on-site evaluation


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Ross A. Miller
    • 1
  • Ashwyna Sunassee
    • 2
  1. 1.Memorial Pathology Consultants, P.AKingwoodUSA
  2. 2.Department of Pathology and Genomic MedicineHouston Methodist HospitalHoustonUSA

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