Chitosan (medium molecular weight (MMW)) with a degree of deacetylation of about 80.0%), chitosan (high molecular weight (HMW)) and alginic acid sodium salt from brown algae (medium molecular weight) were obtained from Sigma-Aldrich Co. LLC. Acetic acid was supplied from Ajax Finechem and used directly without further purification. Calcium chloride fused dihydrate (molecular weight of 147.02 obtained from Chem-supply) was used as the coagulating agent.
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