Comparison of Porcine Brain Tissue with Potential Surrogate Materials Under Quasi-static Compression and Dynamic Mechanical Analysis
Computational models and physical surrogates can provide important insight into complex biomechanical loadings, such as those leading to mild Traumatic Brain Injury (mTBI). However, accurate material properties are required in order to fully realize the potential of such models. This study compares the material properties of porcine brain tissue with various surrogate materials (0.4%, 0.6%, 0.8% concentration agarose gelatin; 3%, 5%, 10% concentration bovine ballistic gelatin; and Sylgard 527). The quasi-static compression properties and dynamic viscoelastic properties (1–200 Hz) for each material were measured using a Dynamic Mechanical Analyzer (TA Instruments, DMA Q800). The tests were performed at room temperature, using fresh porcine brain tissue samples (less than 15 min post-mortem) obtained from a local abattoir. The quasi-static compression stress-strain curves of 5% concentration was in good agreement with the experimental data, while the remaining tissue surrogates were generally higher in stiffness. In terms of dynamic analysis, the complex modulus of the agar and Sylgard 527 materials were found to be comparable to the porcine brain tissue, while the bovine gelatin diverged at higher frequencies.
KeywordsBrain tissue Gelatin DMA Material properties Tissue simulants
In many injurious conditions, such as mild Traumatic Brain Injury (mTBI), the complexity of the biomechanical loading creates challenges for researchers to understand and mitigate the mechanisms of injury. In such cases, computational models  and physical surrogates  may provide important insights to aid in understanding injury and developing protection systems. However, both of these are often limited by the biofidelity of the constitutive model or tissue simulant materials used to assess response. Thus, there is a need for accurate material properties of brain tissue to support computational modeling, as well as the identification of potential tissue simulants that can be used in physical test surrogates.
Previous studies in the literature have identified some common brain tissue simulants, most commonly gelatins (porcine and bovine) , hydrogels , and silicon elastomers . This study measures the response of fresh porcine brain tissue and a variety of current tissue simulants in quasi-static compression and using Dynamic Mechanical Analysis (DMA).
The use of animal tissues in this study was approved by the University of Waterloo Office of Research Ethics (UW ORE: A-14-11), and all tissue handling and disposal was done in accordance with their guidelines. Fresh porcine brains were sourced from a local abattoir, and obtained within 15 min post-mortem. Tissue samples were prepared and tested within 4 h to prevent degradation of the tissue. The samples were mixed gray and white matter and were excised using a scalpel. In addition, three different tissue simulant materials were tested: bovine gelatin (at 3%, 5%, and 10% weight concentration), agarose gel (at 0.4%, 0.6%, and 0.8% weight concentration), and Sylgard 527. All tests were performed at room temperature (25 °C).
The materials testing was performed using a Dynamic Mechanical Analyzer (DMA, TA Instruments Q800). Each material was tested in quasi-static compression and dynamic sinusoidal compression. The quasi-static compression tests provided stress-strain data for each material. The dynamic tests provided storage and loss moduli over a range of frequencies (1–200 Hz).
The properties of fresh porcine brain tissue and three tissue simulants were measured in quasi-static compression and dynamic mechanical analysis. The compressive stress-strain response of 5% bovine gelatin was found to most closely match the response the brain tissue, while the agar gels and Sylgard 527 were observed to exhibit higher stiffness. With respect to dynamic response, the bovine gelatins were observed to have significantly greater complex moduli at higher frequencies, whereas the other simulants were comparable to the measured brain tissue response.
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