Advertisement

Condensation and Decomposition of Nucleotides in Simulated Hydrothermal Fields

  • Ryan Lorig-Roach
  • David Deamer
Chapter
Part of the Nucleic Acids and Molecular Biology book series (NUCLEIC, volume 35)

Abstract

This chapter describes recent studies in which nucleic acid oligomers are synthesized in simulated hydrothermal field conditions using cycles of dehydration and rehydration to promote ester bond synthesis. Such conditions involve elevated temperatures and acidic pH ranges that are also conducive to depurination of nucleotides. For this reason it is important to establish the extent to which depurination occurs and whether this limits yields of oligomers. Here we review condensation reactions that occur in mixtures of AMP and UMP undergoing multiple dehydration cycles in acidic conditions, and report new results related to depurination under the same conditions. Although depurination could be detected, the reaction was inhibited by the presence of a phospholipid. Furthermore, a fraction of the original AMP remains in subsequent cycles, suggesting that depurination does not proceed to completion. We conclude that even though decomposition of mononucleotides occurs in hydrothermal cycling, purine nucleotides will continue to be available to participate in polymerization.

References

  1. Da Silva L, Maurel M-C, Deamer D (2015) Salt-promoted synthesis of RNA-like molecules in simulated hydrothermal conditions. J Mol Evol 80:86–97CrossRefPubMedGoogle Scholar
  2. Damer B, Deamer D (2015) Coupled phases and combinatorial selection in fluctuating hydrothermal pools: a scenario to guide experimental approaches to the origin of cellular life. Life (Basel) 5:872–887.  https://doi.org/10.3390/life5010872CrossRefGoogle Scholar
  3. Deamer D, Singaram S, Rajamani S, Kompanichenko V, Guggenheim S (2006) Self-assembly processes in the prebiotic environment. Philos Trans R Soc Lond B 361:1809–1818CrossRefGoogle Scholar
  4. DeGuzman V, Vercoutere W, Shenasa H, Deamer D (2014) Generation of oligonucleotides under hydrothermal conditions by non-enzymatic polymerization. J Mol Evol 78:251–262CrossRefPubMedGoogle Scholar
  5. Lindahl T (1993) Instability and decay of the primary structure of DNA. Nature 362:709–715CrossRefPubMedGoogle Scholar
  6. Mungi CV, Rajamani S (2015) Characterization of RNA-like oligomers from lipid-assisted nonenzymatic synthesis: implications for origin of informational molecules on early earth. Life (Basel) 5:65–84Google Scholar
  7. Rajamani S, Vlassov A, Benner S, Coombs A, Olasagasti F, Deamer D (2008) Lipid-assisted synthesis of RNA-like polymers from mononucleotides. Orig Life Evol Biosph 38:57–74CrossRefPubMedGoogle Scholar
  8. Rios AC, Yua HT, Tor Y (2015) Hydrolytic fitness of N-glycosyl bonds: comparing the deglycosylation kinetics of modified, alternative, and native nucleosides. J Phys Org Chem 28(3):173–180CrossRefPubMedGoogle Scholar
  9. Stockbridge RB, Schroeder GK, Wolfenden R (2010) The rate of spontaneous cleavage of the glycosidic bond of adenosine. Bioorg Chem 38:224–228CrossRefPubMedPubMedCentralGoogle Scholar
  10. Szostak JW, Bartel DP, Luisi PL (2001) Synthesizing life. Nature 409:387–390CrossRefPubMedGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biomolecular EngineeringUniversity of CaliforniaSanta CruzUSA

Personalised recommendations