Laboratory Diagnosis of HBV

  • Şükran Köse
  • Tuba Dal


Hepatitis B virus (HBV) is an enveloped virus with a genome composed by partially double-stranded DNA. The diagnosis of HBV infection is generally based on serological and molecular assays. Enzyme immunoassays are the most important serological assays identifying virus-encoded antigens and their corresponding antibodies. The molecular tests focus on the detection of quantitative viral load, drug resistance mutations, genotyping, and core promotion/precore mutation assays. The fully automated real-time polymerase chain reaction (PCR) methods are considered as standard methods to detect and quantify HBV DNA because of their high capacity to detect a wide dynamic range of viral load and due to lack of carry-over contamination. Biosensors and biochips seem to be useful technologies for serological diagnosis of HBV. Ultra-deep sequencing technologies will be useful for the analysis of HBV mutants in order to study the dynamics of viral variants.


Hepatitis B virus Enzyme immunoassays Real-time PCR Sequencing 


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Şükran Köse
    • 1
  • Tuba Dal
    • 2
  1. 1.Department of Infectious Diseases and Clinical MicrobiologyUniversity of Health Science, Tepecik Training and Research HospitalİzmirTurkey
  2. 2.Department of Medical MicrobiologyAnkara Yildirim Beyazıt University Medical FacultyAnkaraTurkey

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