Carbon Radiochemicals (14C) and Stable Isotopes (13C): Crucial Tools to Study Plant-Soil Interactions in Ecosystems

  • Geneviève ChiapusioEmail author
  • Dorine Desalme
  • Philippe Binet
  • François Pellissier


The study of plant-environment interactions has grown steadily during the past two decades. This trend will continue as many environmental changes impact the functioning of ecosystems. One aspect of studying the plant-environment interactions is to focus on the way plants react to abiotic or biotic stresses including chemical mediation between plants or plants-microorganisms (allelopathy) and plant reaction to pollutants. This chapter proposes to focus on carbon radiochemicals (14C) and stable carbon isotope tracers (13C). Indeed, they are powerful techniques in plant ecophysiological researches to describe the transfer and effects of allelochemicals and pollutants in plants and their environment.


  1. Andersen CP (2003) Source-sink balance and carbon allocation below ground in plants exposed to ozone. New Phytol 157:213–228CrossRefGoogle Scholar
  2. Bahn M, Lattanzi FA, Hasibeder R, Wild B, Koranda M, Danese V, Brüggemann N, Schmitt M, Siegwolf R, Richter A (2013) Responses of belowground carbon allocation dynamics to extended shading in mountain grassland. New Phytol 198:116–126CrossRefPubMedPubMedCentralGoogle Scholar
  3. Blessing CH, Werner RA, Siegwolf R, Buchmann N (2015) Allocation dynamics of recently fixed carbon in beech saplings in response to increased temperatures and drought. Tree Physiol 35:585–598CrossRefPubMedGoogle Scholar
  4. Brüggemann N, Gessler A, Kayler Z, Keel SG, Badeck F, Barthel M, Boeckx P, Buchmann N, Brugnoli E, Esperschütz J, Gavrichkova O, Ghashghaie J, Gomez-Casanovas N, Keitel C, Knohl A, Kuptz D, Palacio S, Salmon Y, Uchida Y, Bahn M (2011) Carbon allocation and carbon isotope fluxes in the plant-soil-atmosphere continuum: a review. Biogeosciences 8:3457–3489CrossRefGoogle Scholar
  5. Cebron A, Louvel B, Faure P, France-Lanord C, Chen Y, Murrell JC, Leyval C (2011) Root exudates modify bacterial diversity of phenanthrene degraders in PAH-polluted soil but not phenanthrene degradation rates. Environ Microbiol 13:722–736CrossRefPubMedGoogle Scholar
  6. Cennerazzo J, de Junet A, Audinot JN, Leyval C (2017) Dynamics of PAHs and derived organic compounds in a soil-plant mesocosm spiked with 13C-phenanthrene. Chemosphere 168:1619–1627CrossRefPubMedGoogle Scholar
  7. Chiapusio G, Pellissier F (2001) Methodological setup to study allelochemical translocation in radish seedlings. J Chem Ecol 27:1701–1712CrossRefPubMedGoogle Scholar
  8. Chiapusio G, Pellissier F, Gallet C (2004) Uptake and translocation of phytochemical 2-benzoxazolinone (BOA) in radish seeds and seedlings. J Exp Bot 55:1587–1592CrossRefPubMedGoogle Scholar
  9. Chiapusio G, Gallet C, Dobremez JF, Pellissier F (2005) Allelochemicals: tomorrow’s herbicides? In: Regnault-Roger C, Philogène B Jr, Vincent C (eds) Biopesticides of plant origin. Intercept Ltd, Curepipe, pp 139–151Google Scholar
  10. Desalme D, Binet P, Epron D, Bernard N, Gilbert D, Toussaint ML, Plain C, Chiapusio G (2011a) Atmospheric phenanthrene pollution modulates carbon allocation in red clover (Trifolium pratense L.). Environ Pollut 159:2759–2765CrossRefPubMedGoogle Scholar
  11. Desalme D, Binet P, Bernard N, Gilbert D, Toussaint ML, Chiapusio G (2011b) Atmospheric phenanthrene transfer and effects on two grassland species and their root symbionts: a microcosm study. Environ Exp Bot 71:146–151CrossRefGoogle Scholar
  12. Desalme D, Priault P, Gerant D, Dannoura M, Maillard P, Plain C, Epron D (2017) Seasonal variations drive short-term dynamics and partitioning of recently assimilated carbon in the foliage of adult beech and pine. New Phytol 213:140–153CrossRefPubMedGoogle Scholar
  13. Epron D, Bahn M, Derrien D, Lattanzi FA, Pumpanen J, Gessler A, Högberg P, Maillard P, Dannoura M, Gérant D, Buchmann N (2012) Pulse-labelling trees to study carbon allocation dynamics: a review of methods, current knowledge and future prospects. Tree Physiol 32:776–798CrossRefPubMedGoogle Scholar
  14. Griffis TJ (2013) Tracing the flow of carbon dioxide and water vapor between the biosphere and atmosphere: a review of optical isotope techniques and their application. Agric For Meteorol 174:85–109CrossRefGoogle Scholar
  15. Hartmann H, Trumbore S (2016) Understanding the roles of nonstructural carbohydrates in forest trees - from what we can measure to what we want to know. New Phytol 211:386–403CrossRefPubMedGoogle Scholar
  16. Hartmann H, McDowell NG, Trumbore S (2015) Allocation to carbon storage pools in Norway spruce saplings under drought and low CO2. Tree Physiol 35:243–252CrossRefPubMedGoogle Scholar
  17. Heinrich S, Dippold MA, Werner C, Wiesenberg GLB, Kuzyakov Y, Glaser B (2015) Allocation of freshly assimilated carbon into primary and secondary metabolites after in situ 13C pulse labelling of Norway spruce (Picea abies). Tree Physiol 35:1176–1191PubMedGoogle Scholar
  18. Högberg MN, Briones MJI, Keel SG, Metcalfe DB, Campbell C, Midwood AJ, Thornton B, Hurry V, Linder S, Näsholm T, Högberg P (2010) Quantification of effects of season and nitrogen supply on tree below-ground carbon transfer to ectomycorrhizal fungi and other soil organisms in a boreal pine forest. New Phytol 187:485–493CrossRefPubMedGoogle Scholar
  19. Kasurinen A, Biasi C, Holopainen T, Rousi M, Maenpaa M, Oksanen E (2012) Interactive effects of elevated ozone and temperature on carbon allocation of silver birch (Betula pendula) genotypes in an open-air field exposure. Tree Physiol 32:737–751CrossRefPubMedGoogle Scholar
  20. Kerstel E, Gianfrani L (2008) Advances in laser-based isotope ratio measurements: selected applications. Appl Phys B Lasers Opt 92:439–449CrossRefGoogle Scholar
  21. Kuzyakov Y, Gavrichkova O (2010) REVIEW: time lag between photosynthesis and carbon dioxide efflux from soil: a review of mechanisms and controls. Glob Chang Biol 16:3386–3406CrossRefGoogle Scholar
  22. Plain C, Gérant D, Maillard P, Dannoura M, Dong YW, Zeller B, Priault P, Parent F, Epron D (2009) Tracing of recently assimilated carbon in respiration at high temporal resolution in the field with a tuneable diode laser absorption spectrometer after in situ 13CO2 pulse labelling of 20-year-old beech trees. Tree Physiol 29:1433–1445CrossRefPubMedGoogle Scholar
  23. Ruehr NK, Offermann CA, Gessler A, Winkler JB, Ferrio JP, Buchmann N, Barnard RL (2009) Drought effects on allocation of recent carbon: from beech leaves to soil CO2 efflux. New Phytol 184:950–961CrossRefPubMedGoogle Scholar
  24. Streit K, Rinne KT, Hagedorn F, Dawes MA, Saurer M, Hoch G, Werner RA, Buchmann N, Siegwolf RTW (2013) Tracing fresh assimilates through Larix decidua exposed to elevated CO2 and soil warming at the alpine treeline using compound-specific stable isotope analysis. New Phytol 197:838–849CrossRefPubMedGoogle Scholar
  25. Warren JM, Iversen CM, Garten CT, Norby RJ, Childs J, Brice D, Evans RM, Gu L, Thornton P, Weston DJ (2012) Timing and magnitude of C partitioning through a young loblolly pine (Pinus taeda L.) stand using 13C labeling and shade treatments. Tree Physiol 32:799–813CrossRefPubMedGoogle Scholar
  26. Zang U, Goisser M, Grams TEE, Haberle KH, Matyssek R, Matzner E, Borken W (2014) Fate of recently fixed carbon in European beech (Fagus sylvatica) saplings during drought and subsequent recovery. Tree Physiol 34:29–38CrossRefPubMedGoogle Scholar

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© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Geneviève Chiapusio
    • 1
    • 2
    Email author
  • Dorine Desalme
    • 3
  • Philippe Binet
    • 1
  • François Pellissier
    • 4
  1. 1.Université Bourgogne Franche Comté, CNRS, Chrono-Environnement UMR 6249Montbéliard cedexFrance
  2. 2.Université Savoie Mont Blanc, INRA, CARRTELThonon-les-BainsFrance
  3. 3.Université Lorraine, INRA, Ecologie et Ecophysiologie ForestièresVandoeuvre-lès-NancyFrance
  4. 4.Université Savoie Mont Blanc, CNRS, LECALe Bourget-du-Lac CedexFrance

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