Advertisement

Plant Programmed Cell Death (PCD): Using Cell Morphology as a Tool to Investigate Plant PCD

  • T. John Conway
  • Paul F. McCabe
Chapter

Abstract

Programmed cell death (PCD) is a form of cellular suicide that serves to eliminate damaged or unwanted cells. In plants PCD operates from embryogenesis to the death of the whole plant and is an integral component of both plant defence and development. Studying the regulatory mechanisms of PCD in vivo can be difficult as often cells undergoing PCD are buried beneath living tissue or it is only possible to observe the cells post mortem. For many of the hallmark characteristics there is only a finite window of opportunity to detect their presence and even then, the presence of these markers is only enough to give a qualitative and not a quantitative measure of PCD. To examine the subtle role environmental factors may have on altering PCD activation thresholds it is necessary to quantitatively assess rates and timing of PCD events.

References

  1. Bohra A, Jha UC, Adhimoolam P, Bisht D, Singh NP (2016) Cytoplasmic male sterility (CMS) in hybrid breeding in field crops. Plant Cell Rep 35:967–993CrossRefPubMedGoogle Scholar
  2. Doyle SM, Diamond M, McCabe PF (2010) Chloroplast and reactive oxygen species involvement in apoptotic-like programmed cell death in Arabidopsis suspension cultures. J Exp Bot 61:473–482CrossRefPubMedGoogle Scholar
  3. Hogg BV, Kacprzyk J, Molony EM, O’Reilly C, Gallagher TF, Gallois P, McCabe PF (2011) An in vivo root hair assay for determining rates of apoptotic-like programmed cell death in plants. Plant Methods 7:45CrossRefPubMedPubMedCentralGoogle Scholar
  4. Kacprzyk J, Daly CT, McCabe PF (2011) The botanical dance of death. Programmed cell death in plants. Adv Bot Res 60:169–261CrossRefGoogle Scholar
  5. Kacprzyk J, Dauphinee AN, Gallois P, Gunawardena A, McCabe PF (2016) Methods to study plant programmed cell death. In: Puthalakath H, Hawkins C (eds) Programmed cell death: methods and protocols. Methods in molecular biology, vol 1419. Springer, New York, pp 145–160CrossRefGoogle Scholar
  6. Kacprzyk J, Brogan NP, Daly CT, Doyle SM, Diamond M, Molony EM, McCabe PF (2017) The retraction of the protoplast during PCD is an active, and interruptible, calcium-flux driven process. Plant Sci 260:50–59CrossRefPubMedGoogle Scholar
  7. McCabe PF, Levine A, Meijer P-J, Tapon NA, Pennell RI (1997) A programmed cell death pathway activated in carrot cells cultured at low cell density. Plant J 12:267–280CrossRefGoogle Scholar
  8. Morel JB, Dangl JL (1997) The hypersensitive response and the induction of cell death in plants. Cell Death Differ 4:671–683CrossRefPubMedGoogle Scholar
  9. Reape TJ, McCabe PF (2008) Apoptotic-like programmed cell death in plants. New Phytol 180:13–26CrossRefPubMedGoogle Scholar
  10. Rogers HJ (2006) Programmed cell death in floral organs: how and why do flowers die? Ann Bot 97:309–315CrossRefPubMedPubMedCentralGoogle Scholar
  11. Xu Z, Jiang Y, Zhou G (2015) Response and adaptation of photosynthesis, respiration, and antioxidant systems to elevated CO2 with environmental stress in plants. Front Plant Sci 6:1–17Google Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Biology and Environmental ScienceUniversity College DublinDublinIreland

Personalised recommendations