Systems Approaches to Map In Vivo RNA–Protein Interactions in Arabidopsis thaliana
Proteins that specifically interact with mRNAs orchestrate mRNA processing steps all the way from transcription to decay. Thus, these RNA-binding proteins represent an important control mechanism to double check which proportion of nascent pre-mRNAs is ultimately available for translation into distinct proteins. Here, we discuss recent progress to obtain a systems-level understanding of in vivo RNA–protein interactions in the reference plant Arabidopsis thaliana using protein-centric and RNA-centric methods as well as combined protein binding site and structure probing.
KeywordsCLIP iCLIP mRNA interactome RNA-binding protein RNA immunoprecipitation RNA–protein interaction
The work in Tino Köster’s lab is supported by the DFG through grant KO 5364/1-1. Martin Lewinski is supported by the DFG through grant STA653/6-1 to Dorothee Staiger.
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