Biophysical Analysis of miRNA-Dependent Gene Regulation

  • Andrea RibaEmail author
  • Matteo Osella
  • Michele Caselle
  • Mihaela ZavolanEmail author
Part of the RNA Technologies book series (RNATECHN)


microRNAs (miRNAs) are short (∼22 nucleotides long) RNAs that are encoded in the genome of species ranging from viruses to man. Together with proteins of the Argonaute family, they form RNA-induced silencing complexes, which bind target mRNAs, reducing their stability and translation rate. A miRNA typically has hundreds of evolutionarily conserved binding sites across the transcriptome, and frequently, a given mRNA carries binding sites for multiple miRNAs. In this chapter we discuss behaviors that miRNA-containing regulatory networks can exhibit, with specific examples from various experimental systems.


microRNA Ultrasensitivity Noise Competing endogenous RNA Binding hierarchy 


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institut de Génétique et Biologie Moléculaire et CellulaireIllkirchFrance
  2. 2.Dipartimento di FisicaTorinoItaly
  3. 3.Biozentrum, University of BaselBaselSwitzerland

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