Development of Assays to Study Angiogenesis
The study of angiogenesis in living mammalian specimens first become possible in 1924 when Sandison developed a transparent chamber that could be implanted in the rabbit ear (Sandison 1924). This enabled direct observation of the formation of new blood vessels in a healing wound. Folkman work started with perfused organs and then moved in vivo, first using the rat air sac model (Folkman et al. 1963, 1971). As he said: “The phenomenon of angiogenesis was largely inaccessible to study. Thus throughout the middle 1970s, we were preoccupied with devising new techniques that would allow angiogenic phenomena to be quantified and permit angiogenesis to be resoved into is subcomponents (Fig. 2.1).” (Folkman 1985a, b).
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