Propagation of the Virus In Vitro

  • Shawn Babiuk


Capripoxviruses comprising sheeppox, goatpox and lumpy skin disease virus (LSDV) have a tropism for epithelial cells. This tropism allows capripoxviruses to be propagated in a wide variety of cell types from cattle, goat and sheep origin with virus titres up to a titre of 106 TCID50 per ml. These cell types include cells from tissues, including the kidney, testes, adrenal, thyroid, skin and muscle. There are currently no cell culture systems which can differentiate lumpy skin disease from sheeppox and goatpox. The tropism and the cytopathic effect for all the evaluated cells are identical between capripoxviruses.


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Shawn Babiuk
    • 1
    • 2
  1. 1.Canadian Food Inspection Agency, National Centre for Foreign Animal DiseaseWinnipegCanada
  2. 2.University of ManitobaWinnipegCanada

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