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Commercial Applications of SCNT in Livestock

  • Mark Walton
Chapter

Abstract

For most people, the report of the birth of Dolly the sheep in 1996 was their first inclination that cloning animals was not science fiction. Dolly’s arrival was both a seminal moment in the science of reproduction and another step in the evolution of Advanced Reproduction Technologies (ART) in livestock. Somatic cell nuclear transfer (SCNT, aka “cloning”), the process that led to Dolly, is a form of ART that results in exact genetic copies of the donor animal. Livestock produced through cloning are no different than any other animal, and livestock products, e.g., meat and milk, from cloned animals, were found by regulators from Europe, Japan, and the United States to be identical to products from conventional animals. Companies in China, Australia, South America, and the United States provide cloning services to livestock producers and breeders. Cost, burdensome regulatory processes, and public antipathy, especially in Europe, have limited the impact of cloning.

Keywords

Clone Clones Cloning Somatic cell nuclear transfer SCNT Advance reproduction technologies ART Dolly Dolly the sheep Livestock Livestock cloning 

References

  1. Baer D (2015) Inside the Korean lab that has cloned more than 600 dogs. Business Insider AustraliaGoogle Scholar
  2. Baguisi A, Behboodi E, Melican DT, Pollock JS, Destrempes MM, Cammuso C et al (1999) Production of goats by somatic cell nuclear transfer. Nat Biotechnol 17(5):456–461.  https://doi.org/10.1038/8632 CrossRefPubMedGoogle Scholar
  3. Barnes FL, Westhusin ME, & Looney CR (1990) Embryo cloning: principles and progress. EdinburghGoogle Scholar
  4. Briggs R, King TJ (1952) Transplantation of living nuclei from blastula cells into enculeated frogs eggs. Proc Natl Acad Sci U S A 38(5):455–463CrossRefPubMedPubMedCentralGoogle Scholar
  5. Carlson DF, Lancto CA, Zang B, Kim ES, Walton M, Oldeschulte D et al (2016) Production of hornless dairy cattle from genome-edited cell lines. Nat Biotechnol 34(5):479–481.  https://doi.org/10.1038/nbt.3560 CrossRefPubMedGoogle Scholar
  6. Carlson DF, Tan W, Lillico SG, Stverakova D, Proudfoot C, Christian M et al (2012) Efficient TALEN-mediated gene knockout in livestock. Proc Natl Acad Sci U S A 109(43):17382–17387.  https://doi.org/10.1073/pnas.1211446109 CrossRefPubMedPubMedCentralGoogle Scholar
  7. Chavatte-Palmer P, Camous S, Jammes H, Le Cleac'h N, Guillomot M, Lee RS (2012) Review: placental perturbations induce the developmental abnormalities often observed in bovine somatic cell nuclear transfer. Placenta 33(Suppl):S99–s104.  https://doi.org/10.1016/j.placenta.2011.09.012 CrossRefPubMedGoogle Scholar
  8. Cohen H (2015) How champion-pony clones have transformed the game of Polo. Vanity FairGoogle Scholar
  9. Department of Animal Science, U. o. W (2000) HIstory of artificial insemination. Retrieved from http://www.ansci.wisc.edu/jjp1/ansci_repro/lec/handouts/hd8.html
  10. Dominiek M, Alfonso LR, Tom R, Philip V, Ann VS (2011) Artificial insemination in pigs, artificial insemination in farm animals milad manafi. IntechOpen, London.  https://doi.org/10.5772/16592 Available from: https://www.intechopen.com/books/artificial-insemination-in-farm-animals/artificial-insemination-in-pigs CrossRefGoogle Scholar
  11. EFSA (2009) Statement of EFSA prepared by the scientific committee and advisory forum unit on further advice on the implications of animal cloning (SCNT). EFSA J 319:1–15Google Scholar
  12. Estienne MJ, & Harper AF (2009) Using artificial insemination in swine production: detecting and synchronizing estrus and using proper insemination technique, 8. Retrieved from www.ext.vt.edu
  13. Faber D, Ferre LB, Metzger J, Robl J, Kasinathan P (2004) Agro-economic impact of cattle cloning. Cloning Stem Cells 6(2):198–206CrossRefPubMedGoogle Scholar
  14. Faber DC, Molina JA, Ohlrichs CL, Zwaag DFV, Ferre LB (2003) Commercialization of animal biotechnology. Theriogenology 59:125–138CrossRefPubMedGoogle Scholar
  15. FDA. (2008). Animal cloning: a risk assessment. Center for Veterinary Medicine, U.S. Food and Drug Administration. Rockville, MDGoogle Scholar
  16. FDA, U. S. (2009) FDA approves orphan drug atryn to treat rare clotting disorder [Press release]. Retrieved from http://www.fda.gov/NewsEvents/Newsroom/PressAnnouncements/ucm109074.htm
  17. Fiester A (2005) Ethical issues in animal cloning. Perspect Biol Med 48(2):328–343CrossRefPubMedGoogle Scholar
  18. Foote RH (2002) The history of artificial insemination: selected notes and notables. J Anim Sci 80(E-Suppl_2):1–10.  https://doi.org/10.2134/animalsci2002.80E-Suppl_21a CrossRefGoogle Scholar
  19. Galli C, Lagutina I, Crotti G, Colleoni S, Turini P, Ponderato N et al (2003) Pregnancy: a cloned horse born to its dam twin. Nature 424(6949):635–635.  https://doi.org/10.1038/424635a CrossRefPubMedGoogle Scholar
  20. Godke RA, Denniston RS, Reggio B (2003) Animal biotechnology and the future. Louisiana Agric 46(4):10–14Google Scholar
  21. Gordon JW, Ruddle FH (1981) Integration and stable germ line transmission of genes injected into mouse pronuclei. Science 214(4526):1244–1246CrossRefPubMedGoogle Scholar
  22. Granleese T, Clark SA, Swan AA, van der Werf JH (2015) Increased genetic gains in sheep, beef and dairy breeding programs from using female reproductive technologies combined with optimal contribution selection and genomic breeding values. Genet Sel Evol 47:70.  https://doi.org/10.1186/s12711-015-0151-3 CrossRefPubMedPubMedCentralGoogle Scholar
  23. Gurdon JB (1962) The developmental capacity of nuclei taken from intestinal epithelium cells of feeding tadpoles. J Embryol Exp Morphol 10(4):622–640PubMedGoogle Scholar
  24. Hansen PJ (2006) Realizing the promise of IVF in cattle–an overview. Theriogenology 65(1):119–125.  https://doi.org/10.1016/j.theriogenology.2005.09.019 CrossRefPubMedGoogle Scholar
  25. Hasler JF (2003) The current status and future of commercial embryo transfer in cattle. Anim Reprod Sci 79(3–4):245–264CrossRefPubMedGoogle Scholar
  26. Hawkins D, & Lawrence T (2013) From imagination to reality: using DNA from an exceptional carcass to produce a sire or donor cow. paper presented at the range beef cow symposium, Rapid City, SD. http://digitalcommons.unl.edu/rangebeefcowsymp/309
  27. Hodges CA, Stice SL (2003) Generation of bovine transgenics using somatic cell nuclear transfer. Reprod Biol Endocrinol 1:81.  https://doi.org/10.1186/1477-7827-1-81 CrossRefPubMedPubMedCentralGoogle Scholar
  28. Infigen (2001) World's first herd of cloned dairy cows in production at infigen [Press release]Google Scholar
  29. Intrexon (2016) Game-changing animal research models offer superior translational research and better predictive efficacy [Press release]. Retrieved from http://investors.dna.com/2016-04-27-Game-Changing-Animal-Research-Models-Offer-Superior-Translational-Research-and-Better-Predictive-Efficacy
  30. JFSC (2009) Risk assessment report on foods derived from cloned cattle and pigs produced by somatic cell nuclear transfer (SCNT) and their offspring (Novel Foods)Google Scholar
  31. Kasinathan, P., Wei, H., Xiang, T., Molina, J. A., Metzger, J., Broek, D., . . . Allan, M. F. (2015). Acceleration of genetic gain in cattle by reduction of generation interval. Sci Rep, 5, 8674. doi: https://doi.org/10.1038/srep08674 CrossRefPubMedPubMedCentralGoogle Scholar
  32. Kato Y, Tani T, Sotomaru Y, Kurokawa K, Kato J-y, Doguchi H et al (1998) Eight calves cloned from somatic cells of a single adult. Science 282(5396):2095–2098.  https://doi.org/10.1126/science.282.5396.2095 CrossRefPubMedGoogle Scholar
  33. Khanal AR, Gillespie J (2013) Adoption and productivity of breeding technologies: evidence from U.S. dairy farms. AgBioforum 16(1):53–65Google Scholar
  34. Kinghorn B (2000) Animal production and breeding systems to exploit cloning technology. In: Kinghorn BP, Van der Werf JH, Ryan M (eds) Animal breeding: use of new technologies. University of Sydney: Post Graduate Foundation in Veterinary Science, University of Sydney, SydneyGoogle Scholar
  35. Lai L, Prather RS (2003) Creating genetically modified pigs by using nuclear transfer. Reprod Biol Endocrinol 1:82–82.  https://doi.org/10.1186/1477-7827-1-82 CrossRefPubMedPubMedCentralGoogle Scholar
  36. Lee K, Prather RS (2013) Advancements in somatic cell nuclear transfer and future perspectives. Anim Front 3(4):56–61.  https://doi.org/10.2527/af.2013-0034 CrossRefGoogle Scholar
  37. Liu J, Wang Y, Su J, Luo Y, Quan F, Zhang Y (2013) Nuclear donor cell lines considerably influence cloning efficiency and the incidence of large offspring syndrome in bovine somatic cell nuclear transfer. Reprod Domest Anim 48(4):660–664.  https://doi.org/10.1111/rda.12140 CrossRefPubMedGoogle Scholar
  38. Loi P, Ptak G, Barboni B, Fulka J Jr, Cappai P, Clinton M (2001) Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells. Nat Biotechnol 19(10):962–964.  https://doi.org/10.1038/nbt1001-962 CrossRefPubMedGoogle Scholar
  39. Long CR, Westhusin ME, Golding MC (2014) Reshaping the transcriptional frontier: epigenetics and somatic cell nuclear transfer. Mol Reprod Dev 81(2):183–193.  https://doi.org/10.1002/mrd.22271 CrossRefPubMedGoogle Scholar
  40. Mander B (2013) Polo players look twice at cloned ponies. Financial Times. Retrieved from http://www.ft.com
  41. Mapletoft RJ, Hasler JF (2005) Assisted reproductive technologies in cattle: a review. Rev Sci Tech 24(1):393–403CrossRefPubMedGoogle Scholar
  42. Michel J (2014) US company in Iowa churns out 100 cloned cows a year. The Tico Times. Retrieved from http://www.ticotimes.net/?s=iowa+company+churns+out+100+cloned+cows+a+year
  43. Murray JD, Anderson GG (2000) Genetic engineering and cloning may improve milk, livestock production. Calif Agric 54(4):57–65CrossRefGoogle Scholar
  44. Nicholas W (2013) The clone named dolly. The New York Times. Retrieved from http://www.nytimes.com
  45. Niemann H, Lucas-Hahn A (2012) Somatic cell nuclear transfer cloning: practical applications and current legislation. Reprod Domest Anim 47(Suppl 5):2–10.  https://doi.org/10.1111/j.1439-0531.2012.02121.x CrossRefPubMedGoogle Scholar
  46. NIH (1997) Prohibition on federal funding for cloning of human beings. NIH Grants. Retrieved from https://grants.nih.gov/grants/policy/cloning_directive.htm
  47. Oback B (2008) Climbing mount efficiency–small steps, not giant leaps towards higher cloning success in farm animals. Reprod Domest Anim 43(Suppl 2):407–416.  https://doi.org/10.1111/j.1439-0531.2008.01192.x CrossRefPubMedGoogle Scholar
  48. Palca J (2009) Science Diction: The Origin of the Word ‘Clone’. Accessed at https://www.npr.org/2011/03/11/134459358/Science-Diction-The-Origin-Of-The-Word-Clone
  49. Panarace M, Agüero JI, Garrote M, Jauregui G, Segovia A, Cané L et al (2007) How healthy are clones and their progeny: 5 years of field experience. Theriogenology 67(1):142–151.  https://doi.org/10.1016/j.theriogenology.2006.09.036 CrossRefPubMedGoogle Scholar
  50. Perry G (2017) 2016 statistics of embryo collection and transfer in domestic farm animals. Retrieved from http://www.iets.org/pdf/comm_data/IETS_Data_Retrieval_Report_2016_v2.pdf
  51. Petersen B, Lucas-Hahn A, Oropeza M, Hornen N, Lemme E, Hassel P et al (2008) Development and validation of a highly efficient protocol of porcine somatic cloning using preovulatory embryo transfer in peripubertal gilts. Cloning Stem Cells 10(3):355–362.  https://doi.org/10.1089/clo.2008.0026 CrossRefPubMedGoogle Scholar
  52. Phys.org (2015) World's biggest clone factory raises fears in China. Retrieved from http://phys.org/news/2015-11-world-biggest-animal-clone-factory.html
  53. Plume K (2009) Welcome to the clone farm. Reuters. Retrieved from http://www.reuters.com/article/usfoodcloningidUSTRE5AC07V20091 1 13
  54. Polejaeva IA, Broek DM, Walker SC, Zhou W, Walton M, Benninghoff AD, Faber DC (2013) Longitudinal study of reproductive performance of female cattle produced by somatic cell nuclear transfer. PLoS One 8(12):e84283.  https://doi.org/10.1371/journal.pone.0084283 CrossRefPubMedPubMedCentralGoogle Scholar
  55. Polejaeva IA, Chen S-H, Vaught TD, Page RL, Mullins J, Ball S et al (2000) Cloned pigs produced by nuclear transfer from adult somatic cells. Nature 407:86–90CrossRefPubMedGoogle Scholar
  56. Prather RS, Sims MM, First NL (1989) Nuclear transplantation in early pig embryos. Biol Reprod 41:414–418CrossRefPubMedGoogle Scholar
  57. Rudenko L, Matheson JC (2007) The US FDA and animal cloning: risk and regulatory approach. Theriogenology 67(1):198–206.  https://doi.org/10.1016/j.theriogenology.2006.09.033 CrossRefPubMedGoogle Scholar
  58. Spemann H (1938) Embryonic development and induction. Yale University Press, New HavenGoogle Scholar
  59. Stice SL, Keefer CL (1993) Multiple generational bovine embryo cloning. Biol Reprod 48:715–719CrossRefPubMedGoogle Scholar
  60. Stice SL, Robl J, Ponce de Leon FA, Jerry J, Golueke PG, Cibelli JB, Kane JJ (1998) Cloning: new breakthroughs leading to commercial opportunities. Theriogenology 49:129–138CrossRefPubMedGoogle Scholar
  61. Sunderland ME (2012) Hans Adolf Eduard Driesch (1867–1941) Embryo Project EncyclopediaGoogle Scholar
  62. Tan W, Carlson DF, Lancto CA, Garbe JR, Webster DA, Hackett PB, Fahrenkrug SC (2013) Efficient nonmeiotic allele introgression in livestock using custom endonucleases. Proc Natl Acad Sci U S A 110(41):16526–16531.  https://doi.org/10.1073/pnas.1310478110 CrossRefPubMedPubMedCentralGoogle Scholar
  63. Tan WS, Carlson DF, Walton MW, Fahrenkrug SC, Hackett PB (2012) Precision editing of large animal genomes. Adv Genet 80:37–97.  https://doi.org/10.1016/B978-0-12-404742-6.00002-8 CrossRefPubMedGoogle Scholar
  64. Taylor-Robinson AW, Walton S, Swain DL, Walsh KB, Vajta G (2014) The potential for modification in cloning and vitrification technology to enhance genetic progress in beef cattle in northern Australia. Anim Reprod Sci 148(3–4):91–96.  https://doi.org/10.1016/j.anireprosci.2014.06.004 CrossRefPubMedGoogle Scholar
  65. Thibier M (1998) The 1997 Embryo transfer statistics from around the World. Data Retrieval Committee Report, International Embryo Transfer Society. https://www.iets.org/pdf/comm_data/December1998.pdf. Accessed May 2016
  66. Thomas I (2012) Should scientists pursue cloning? Heinemann-Raintree, ChicagoGoogle Scholar
  67. Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM (1998) Embryonic stem cell lines derived from human blastocysts. Science 282(5391):1145–1147CrossRefGoogle Scholar
  68. U.S. Department of Agriculture, O. o. C (2008) USDA statement on FDA risk assessment on animal clones. USDA. Retrieved from http://www.usda.gov/wps/portal/usda/usdahome?contentidonly=true&contentid=2008/01/0012.xml
  69. Vogel G (2015) E.U. parliament votes to ban cloning of farm animals. Science News. Retrieved from http://www.sciencemag.org/news/2015/09/eu-parliament-votes-ban-cloning-farm-animals
  70. Walton M (2013) Cloning and animal improvement. Paper presented at the impact of biotechnology on future animal breeding, Berlin, GermanyGoogle Scholar
  71. Walton MF (2016) Use of SCNT in production of biomedical pigs. Unpublished Raw DataGoogle Scholar
  72. Wells DN (2005) Animal cloning: problems and prospects. Rev Sci Tech 24(1):251–264CrossRefPubMedGoogle Scholar
  73. Wells DN, Laible G, Tucker FC, Miller AL, Oliver JE, Xiang T et al (2003) Coordination between donor cell type and cell cycle stage improves nuclear cloning efficiency in cattle. Theriogenology 59(1):45–59.  https://doi.org/10.1016/S0093-691X(02)01273-6 CrossRefPubMedGoogle Scholar
  74. Wells DN, Misica PM, Tervit RH (1999) Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells. Biol Reprod 60(4):996–1005.  https://doi.org/10.1095/biolreprod60.4.996 CrossRefPubMedGoogle Scholar
  75. Westhusin ME, Stroud BK, Kraemer DC, & Long CR 2005) Cloning bovine embryos: current status and future applications. Paper presented at the Proceedings, Applied Reproductive Strategies in Beef Cattle, Texas A&M University, College Station, TexasGoogle Scholar
  76. Wheeler MB (2003) Production of transgenic livestock: promise fulfilled. J Anim Sci 81(Suppl. 3):32–37CrossRefPubMedGoogle Scholar
  77. Willadsen SM (1986) Nuclear transplantation in sheep embryos. Nature 320(6057):63–65.  https://doi.org/10.1038/320063a0 CrossRefPubMedGoogle Scholar
  78. Willadsen SM (1989) Cloning of sheep and cow embryos. Genome 31(2):956–962.  https://doi.org/10.1139/g89-167 CrossRefPubMedGoogle Scholar
  79. Williams O (2015) Battle of the clones: when will a replica horse win olympic gold? Retrieved from http://edition.cnn.com/2015/02/20/equestrian/horse-cloning-olympics/index.html
  80. Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KH (1997) Viable offspring derived from fetal and adult mammalian cells. Nature 385(6619):810–813.  https://doi.org/10.1038/385810a0 CrossRefPubMedGoogle Scholar

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Authors and Affiliations

  1. 1.MWalton Enterprises llcAustinUSA

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