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Cloning of Livestock by Somatic Cell Nuclear Transfer

  • Kenneth R. Bondioli
Chapter

Abstract

Since the cloning of “Dolly” by somatic cell nuclear transfer in 1996, numerous articles have been published concerning the application of this technology to a large variety of mammalian species including all the major livestock species. While live births have been obtained for many species, the efficiency of cellular reprogramming essential for success has not been significantly improved. This chapter will attempt to address the inputs utilized for this procedure and the major manipulation steps with the objective of identifying the major factors which might affect this efficiency of reprogramming and some of the studies addressing these factors. Finally, the challenging task of setting optimum endpoints for experiments involving domestic species will be discussed.

Keywords

Cloning Nuclear transfer Livestock Oocytes Donor cells Enucleation Fusion Efficiency 

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Animal SciencesLouisiana State University, Agricultural CenterBaton RougeUSA

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